文章摘要
梁箫,张亚,刘钰珠,李一峰,BATISTA M F,POWER D,杨金龙.灿烂弧菌对厚壳贻贝免疫指标和消化酶活性的影响[J].水产学报,2018,42(9):1438~1445
灿烂弧菌对厚壳贻贝免疫指标和消化酶活性的影响
Influence of Vibrio splendidus on immune indexes and digestive enzyme activity of Mytilus coruscus
投稿时间:2018-02-06  修订日期:2018-04-09
DOI:10.11964/jfc.20180211176
中文关键词: 厚壳贻贝  灿烂弧菌  免疫指标  消化酶
英文关键词: Mytilus coruscus  Vibrio splendidus  immune index  digestive enzyme
基金项目:国家重点研发计划政府间国际科技创新合作重点专项(2016YFE0131900);上海高校水产高峰学科建设项目
作者单位E-mail
梁箫 上海海洋大学国家海洋生物科学国际联合研究中心, 上海 201306
上海海洋大学水产种质资源发掘与利用教育部重点实验室, 上海 201306
上海海洋大学水产科学国家级实验教学示范中心, 上海 201306 
 
张亚 上海海洋大学国家海洋生物科学国际联合研究中心, 上海 201306
上海海洋大学水产种质资源发掘与利用教育部重点实验室, 上海 201306 
 
刘钰珠 上海海洋大学国家海洋生物科学国际联合研究中心, 上海 201306
上海海洋大学水产种质资源发掘与利用教育部重点实验室, 上海 201306 
 
李一峰 上海海洋大学国家海洋生物科学国际联合研究中心, 上海 201306
上海海洋大学水产种质资源发掘与利用教育部重点实验室, 上海 201306
上海海洋大学水产科学国家级实验教学示范中心, 上海 201306 
 
BATISTA M F Centre of Marine Sciences, University of Algarve, Faro, Portugal  
POWER D Centre of Marine Sciences, University of Algarve, Faro, Portugal  
杨金龙 上海海洋大学国家海洋生物科学国际联合研究中心, 上海 201306
上海海洋大学水产种质资源发掘与利用教育部重点实验室, 上海 201306
上海海洋大学水产科学国家级实验教学示范中心, 上海 201306 
jlyang@shou.edu.cn 
摘要点击次数: 615
全文下载次数: 404
中文摘要:
      为探究灿烂弧菌对厚壳贻贝免疫指标和消化酶活性的影响,用1×106、1×107、1×108个/mL 3个浓度的灿烂弧菌刺激厚壳贻贝,探讨弧菌刺激后厚壳贻贝的一氧化氮合酶(NOS)、一氧化氮(NO)、超氧化物歧化酶(SOD)、丙二醛(MAD)等免疫指标和淀粉酶、蛋白酶等消化酶的变化情况。结果显示,灿烂弧菌刺激48 h后,厚壳贻贝足、鳃、消化腺等组织中仅消化腺的NOS活性较对照组有显著升高,且酶活性随初始细菌浓度的升高而升高,故选用消化腺来测定灿烂弧菌刺激后72 h内的免疫指标和消化酶活性的变化。灿烂弧菌刺激后,48 h内NOS活性较对照组均显著升高。NO含量较对照组均显著升高,与NOS显现相同变化趋势。SOD活性在各浓度灿烂弧菌刺激下较对照组均显著升高,而MAD含量在实验组中含量显著低于对照组。淀粉酶活性在实验组中显著低于对照组,总体呈现先下降后升高的趋势。蛋白酶活性在各实验组中均呈现先升高后下降的趋势。研究表明,灿烂弧菌对厚壳贻贝免疫指标和蛋白酶活性的升高有诱导作用,但对蛋白酶的活性有抑制作用。本研究初步探明了厚壳贻贝对灿烂弧菌的免疫应答机制,为进一步研究灿烂弧菌和厚壳贻贝相互作用机制以及厚壳贻贝免疫机制奠定了基础。
英文摘要:
      Mytilus coruscus adults were challenged by Vibrio splendidus in three concentrations (1×106 cell/mL, 1×107 cell/mL, 1×108 cell/mL). The changes of immune indexes including nitric oxide synthase (NOS), nitric oxide (NO), superoxide dismutase (SOD) and malondialdehyde (MAD), and digestive enzyme activities including amylase and protease in digestive gland were determined during 72 h experiment period. The activities of NOS in foot, gill and digestive gland were measured after experimental challenges of 48 h. Only the activity of NOS in the digestive gland was significantly different from the control group, and the activity of NOS increased with the increase of initial concentration of V. splebdidus. The activity of NOS of digestive gland in the treatment group was significantly higher than that of the control group after the challenge of V. splebdidu, and the level of NOS significantly increased after 48 h. The content of NO was significantly higher than that in the control group. The content of NO increased first and then decreased, which had a similar trend with the activity of NOS. The activity of SOD was significantly higher compared with that of the control group after the challenge of V. splebdidu. The content of MAD in the treatment group was significantly lower than that in the control group after the challenge of bacteria. The activity of amylase in the treatment group was significantly lower than that in the control group. The activity of amylase descended first and then increased. The change of protease activity in the treatment group increased first and then decreased. This study indicates that V. splebdidu can promote the immune indexes and protease activity and inhibit amylase activity. The present study provides useful information for clarifying the immune mechanism of M. coruscus and Vibrio-mussel interactions.
HTML   查看全文   下载PDF阅读器
关闭

手机扫一扫看