文章摘要
添加芽孢杆菌对池塘中理化因子和细菌群落结构的影响分析
Analysis of the effect of adding Bacillus on the physicochemical factors and bacterial community structure in ponds
投稿时间:2018-11-02  修订日期:2019-03-11
DOI:
中文关键词: 芽孢杆菌  池塘  水质  高通量测序  细菌群落结构
英文关键词: Bacillus  pond  physical and chemical factors  high-throughput sequencing  bacterial community structure.
基金项目:广州市科技计划项目(201804010163);国家现代农业产业技术体系建设专项(CARS-45-21);广东省省级科技计划项目(2017B090906002);广东省自然科学基金(2018A030313412)
作者单位E-mail
陆洋 中国水产科学研究院珠江水产研究所 www.luyang1992@qq.com 
郁二蒙 中国水产科学研究院珠江水产研究所  
谢骏 中国水产科学研究院珠江水产研究所  
王广军 中国水产科学研究院珠江水产研究所

上海海洋大学

广东省水产养殖污染修复生态工程技术研究中心
广东省水产养殖污染修复生态工程技术研究中心 
gjwang@prfri.ac.cn 
张凯 中国水产科学研究院珠江水产研究所  
余德光 中国水产科学研究院珠江水产研究所  
李志斐 中国水产科学研究院珠江水产研究所  
摘要点击次数: 469
全文下载次数: 0
中文摘要:
      为了研究添加芽孢杆菌对池塘中理化因子和细菌群落结构的影响,本文采用高通量测序技术分析了实验组(添加芽孢杆菌池塘)与对照组(普通池塘)水体和底泥细菌群落结构,同时分析了两种池塘水体和底泥的理化指标。结果表明,8、9月实验组池塘水体中TN、NH4+-N 和NO3--N含量显著低于对照组(P<0.05);但实验组底泥中的NO3--N、NH4+-N、TN和TP含量显著高于对照组(P<0.05)。水体中Chlamydomonas debaryana、发酵单胞菌属(zymomonas)、玫瑰单胞菌(Roseomonas)、脱氯单胞菌(Dechloromonas)和噬几丁质菌属(Chitinophaga)细菌丰度显著高于对照组(P<0.05),在这些细菌群落中,Chlamydomonas debaryana有去除氨氮的功能,脱氯单胞菌、噬几丁质菌属有除去硝酸盐的作用,发酵单胞菌属、玫瑰单胞菌具有脱氮的功能。实验组池塘水体Chao1指数和Shannon指数显著高于对照组(P<0.05)。水体中优势细菌群落中的噬氢菌属(Hydrogenophaga)、Geothermobacter、Haliscomenobacter与硝酸盐、总磷呈负相关性,与氨氮、总氮呈正相关性。实验结果证实通过向池塘添加芽孢杆菌,可以改变水体中细菌群落的结构,从而实现对池塘理化因素的调节。研究结果对于降低水产养殖尾水和水域环境的污染方面具有一定的意义。
英文摘要:
      In order to study the effect of adding Bacillus in the pond on the physicochemical factors and bacterial community structure, high throughput sequencing technology was used to analyze the bacterial community structure in water and sediment of the experimental group (adding Bacillus in pond) and the control group (without adding in pond). At the same time, the physicochemical parameters in water and sediment of the two groups were collected. The results indicated that the contents of TN, NH4+-N, NO3—N in water were significantly lower than those of the control group (P < 0.05), but the contents of NO3--N, NH4+-N, TN and TP in sediment were significantly higher than those of the control group (P < 0.05). The abundance of Chlamydomonas debaryana, Zymomonas,Roseomonas, Dechloromonas and Chitinophaga in water of the experimental group was significantly higher than that in the control group (P<0.05). Among of these bacterial communities, Chlamydomonas debaryana have the function of removing NH4+-N, Dechloromonas and Chitinophaga have the function of removing NO3--N, Zymomonas and Roseomonas have the function of denitrification. The Chao1 and Shannon index in water of the experimental group were significantly higher than those in the control group (P<0.05). Hydrogenophaga, Geothermobacter and Haliscomenobacter were negatively correlated with NO3--N and TP, and positively correlated with NH4+-N and TN. Above all, these results showed that adding Bacillus to the pond would lead to the variations of bacterial community structure, thus realizing the regulation of physicochemical factors in the pond. And the results are meaningful for reducing the pollution of aquaculture tail water and water environment.
HTML     下载PDF阅读器
关闭

手机扫一扫看