文章摘要
中国大鲵幼体蛋白质需求量研究
Dietary Protein Requirement of Juvenile Chinese Giant Salamander(Andrias davidianus)
投稿时间:2018-11-13  修订日期:2019-03-30
DOI:
中文关键词: 中国大鲵  蛋白质  体组成  RNA/DNA比值  消化  抗氧化
英文关键词: Andrias davidianus  protein  body composition  RNA/DNA ratio  digestion  antioxidant
基金项目:
作者单位E-mail
王双 重庆市北碚区西南大学动物科技学院 ws_0407@126.com 
罗莉 西南大学动物科技学院 luoli1972@163.com 
李战福 西南大学动物科技学院  
李伟龙 西南大学动物科技学院  
林仕梅 西南大学动物科技学院  
陈拥军 西南大学动物科技学院  
李虹 重庆市水产技术推广总站  
翟旭亮 重庆市水产技术推广总站  
薛洋 重庆市水产技术推广总站  
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中文摘要:
      (目的)为评定大鲵幼体饲料的蛋白质需要量,(方法)以鱼粉为主要蛋白源配制6种蛋白质水平(干样基础)的实验饲料:D1(43.7%),D2(47.1%),D3(51.3%),D4(55.7%),D5(59.9%)和D6(64.4%),饲喂初始体重为20.99±0.15g的大鲵幼体92天。(结果)结果表明:1)饲料蛋白水平对大鲵增重率有显著影响(P<0.05),在D4组达到最大值,较D1组增加了276.37%,且全鲵蛋白质沉积率和肌肉RNA、RNA/DNA比值、胃蛋白酶、H+/K+-ATPase、胰蛋白酶、脂肪酶和Na+-K+-ATPase、肝脏超氧化物歧化酶(SOD)均在D4组达到最佳,而肝脏和肠道丙二醛(MDA)在该组均达到最低。2)随饲料蛋白水平增加,肌肉粗蛋白线性增加,全鲵脂肪线性下降,全鲵水分和粗灰分在各组间差异不显著(P>0.05),全鲵粗蛋白先增加后趋于稳定,在D4组达到最大。3)大鲵皮肤胶原蛋白含量在D4组达到最高,较D1组增加了27.83%。(结论)以增重率、肌肉RNA/DNA比值、蛋白质沉积率和皮肤胶原蛋白含量为评价指标,通过二次回归方程分析得到大鲵幼体饲料的最适蛋白质水平为55.9%-58.3%(干样基础),该饲料蛋白水平能显著提高大鲵幼体胃的泌酸能力,机体消化吸收和抗氧化能力,增加鲵体营养素的沉积,从而促进生长和饲料的转化;而低蛋白饲料显著抑制大鲵的生长。
英文摘要:
      In order to determine the protein requirement of the diet of juvenile giant salamander, 6 kinds of protein levels (dry matter) were prepared with fish meal as the main protein source: D1(43.7%), D2(47.1%), D3(51.3%), D4(55.7%), D5(59.9%) and D6(64.4%), to feed juvenile giant salamander initially weight 20.99±0.15g for 92 days. The results show that: 1) Dietary protein levels had significant effects on weight gain rate of giant salamander (P < 0.05), it reached the highest in the D4 group, which is 276.37% higher than group D1, and the whole giant salamander protein deposition rate and muscle RNA, RNA/DNA ratio, pepsin, H+/K+ ATPase, trypsin, lipase and Na+/K+ ATPase, liver superoxide dismutase (SOD) reached the best in the D4 group, and the malondialdehyde (MDA) in liver and intestine were both lowest in this group. 2) The content of muscle crude protein linearly with the increase of dietary protein level, while the fat content in giant salamanders declined linearly, and there was no significant difference in moisture and crude ash of whole body between different groups, whole body crude protein increased first and then tended to be stable (P>0.05), reached the highest in the D4 group. 3) The skin collagen content in D4 group reached the highest, increasing by 27.83% compared with D1. With weight gain rate, muscle RNA/DNA ratio, protein deposition, and rate skin collagen content as evaluation indexes, the optimum dietary protein level of the giant salamander was 55.9%-58.3%( based on dry mater), this dietary protein level can significantly improve gastric acid secretion, the digestion and absorption, and antioxidant capacity of the salamander, and increase the nutrient deposition, thus promote growth and feed conversion; While low protein level diet significantly inhibited the growth of Andrias davidianus.
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