文章摘要
三角帆蚌类丝状基质蛋白silkmaxin基因的克隆及其在生物矿化中的作用
The role of the Hyriopsis cumingii shell silk-like matrix protein silkmaxin in the shell and pearl biomineralization
投稿时间:2018-11-21  修订日期:2019-03-30
DOI:
中文关键词: 三角帆蚌  生物矿化  贝壳基质蛋白  类丝状蛋白  珍珠
英文关键词: Hyriopsis cumingii  biomineralization  shell matrix proteins  silk-like proteins  pearl
基金项目:国家自然科学基金(31272657);国家科技支撑计划(2012BAD26B04);现代农业产业技术体系建设专项资金(CARS-49)
作者单位E-mail
夏中慧 上海洋大学农业部淡水产种质资源重点实验室  
刘晓军 上海洋大学农业部淡水产种质资源重点实验室 txliuxj@vip.163.com 
李家乐 上海洋大学农业部淡水产种质资源重点实验室  
摘要点击次数: 518
全文下载次数: 0
中文摘要:
      贝壳基质蛋白指导了珍珠形成过程中碳酸钙的成核、晶体生长和晶型选择等关键过程,是影响珍珠质量的功能基因。为进一步研究珍珠形成的分子机理,本文使用RACE-PCR技术克隆一个新的贝壳基质蛋白基因,并命名为silkmaxin。组织表达分析和原位杂交分析表明该蛋白特异性表达于外套膜缘膜部外上表皮,证明silkmaxin是珍珠层基质蛋白。silkmaxin富含Gly(33.0%)和Ser(10.4%)并由β-折叠构成,蛋白结构类似于丝状蛋白。分析珍珠形成早期珍珠囊中该蛋白基因的表达发现,silkmaxin在珍珠囊内碳酸钙沉积物从无序到有序的转变过程中起了重要作用。通过RNAi干扰研究可知silkmaxin是珍珠层文石小片正常生长不可缺少的因子,当silkmaxin基因表达被抑制,文石小片的成核、大小和形状都发生了改变。
英文摘要:
      Shell matrix proteins control the nuleation、size and crystal phase of calcium carbonate during the biomineralization of pearl, and they are the functional genes related to pearl quality. In order to study more about the molecular mechanism of nacre formation, we cloned a novel shell matrix protein silkmaxin (Accession No. MK188932) from freshwater mussel Hyriopsis cumingii. With the gene expression detected by RT-PCR and in situ hybridization, silkmaxin specially expressed in the pallial outer epithelial cells of outer fold, which indicated that silkmaxin is a nacreous layer matrix protein.The amino acid sequence of silkmaxin featured high proportion of Gly (33.0%) and Ser (10.4%) residues, and the second structure is mainly composed of β-folds. The high structure prediction indicated that silkmaxin is a silk-like protein.In addition, the expression of hic9 in primary pearl sac was detected by real-time quantitative PCR in the early stage of pearl formation. The results indicated that silkmaxin play important roles in the transition of the calcium carbonate disordered deposition to ordered deposition.The study of shell by silkmaxin RNAi treatment indicates that hic9 is essential for the correct growth of aragonite tablets, including the nuleation、size and shape of the newly formed calcium carbonate crystals.
HTML     下载PDF阅读器
关闭

手机扫一扫看