文章摘要
长牡蛎酪氨酸酶基因(CgTyr1)SNP多态性与壳色性状的关联
Polymorphism in CgTyr1 gene and its association with shell color traits in the Pacific oyster Crassostrea gigas
投稿时间:2018-12-03  修订日期:2019-02-25
DOI:
中文关键词: 长牡蛎  壳色  酪氨酸酶基因  SNP  单倍型
英文关键词: Crassostrea gigas  shell color  tyrosinase gene  SNPs  haplotype
基金项目:31,国家自然科学基金
作者单位E-mail
赵博文 中国海洋大学海水养殖教育部重点实验室 zhaobowen.qingdao@outlook.com 
李 琪 中国海洋大学海水养殖教育部重点实验室 qili66@ouc.edu.cn 
王九龙 中国海洋大学海水养殖教育部重点实验室  
于 红 中国海洋大学海水养殖教育部重点实验室  
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中文摘要:
      在动物中,黑色素是一种广泛存在的生物色素,而酪氨酸酶被认为是一种重要的调控黑色素合成的关键酶。本研究以3种壳色的长牡蛎选育品系为材料,使用PCR-SSCP的方法对长牡蛎酪氨酸酶基因CgTyr1进行SNP分型筛选,将突变位点与不同壳色性状进行关联分析。结果显示,酪氨酸酶基因的外显子上存在23个SNP位点,其中11个SNP位点与壳色性状具有极显著相关;在这11个SNP位点中,检测到有3个SNP位点为有义突变(c.591C/T、c.632G/A和c.1155T/C),分别导致不同的氨基酸突变(Ala122Val、Gly136Ser和Phe310Ser);利用极显著关联的11个SNP位点,为每种壳色群体建立了1种单倍型,并在验证组中得到了确认。研究表明长牡蛎酪氨酸酶基因的单个碱基突变和以此构建的单倍型与壳色性状存在显著的关联。本研究筛选出的SNP位点和构建的单倍型为长牡蛎壳色品系选育提供了重要的参考资料。
英文摘要:
      The Pacific oyster Crassostrea gigas is cultured worldwide due to the advantages of rapid growth and good adaptability, and has become one of the most commercially important bivalve species. In our successively selective breeding, three strains of C. gigas with black, white and gold shell color traits have been developed. Tyrosinase (Tyr) is known as one of the most important enzymes in the regulation and production of melanin in animals. In this study, exons in the Pacific oyster TYR gene (CgTyr1) were sequenced. Mutations of the CgTyr1 gene and its association with shell color were analyzed in the three shell color strains of C. gigas. A total of 23 single nucleotide polymorphisms (SNP) were detected using single-strand conformation polymorphism (SSCP) and sequencing analysis, of which 11 SNP loci were highly significant difference between the three shellcolor strains (P<1×10-6). In the SNP loci with significant difference (P<1×10-6), mutation c.591C/T, c.632G/A and c.1155T/C are non-synonymous which lead to amino acid changes Ala122Val, Gly136Ser and Phe310Ser. For further analysis, 11 SNP loci with a highly significant difference (P<1×10-6) were selected for haplotype construction. One specific haplotype for every shell color strain was constructed and confirmed in the validating group. The mutations and haplotypes that are strongly associated with the shell color phenotypes in this study could be useful in understanding the molecular mechanism of pigmentation, and potentially applied to marker-assisted selection breeding programs for C. gigas.
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