文章摘要
魁蚶各组织溶菌酶活性对鳗弧菌侵染的响应
Response of lysozyme activity to Vibrio anguillarum infection in different tissues of Scapharca broughtonii
投稿时间:2018-12-11  修订日期:2019-04-26
DOI:
中文关键词: 魁蚶  溶菌酶  鳗弧菌侵染
英文关键词: Scapharca broughtonii  lysozyme  Vibrio anguillaris infection
基金项目:
作者单位E-mail
赵丹 上海海洋大学
中国水产科学研究院黄海水产研究所 
1282095341@qq.com 
周丽青 中国水产科学研究院黄海水产研究所 zhoulq@ysfri.ac.cn 
吴彪 中国水产科学研究院黄海水产研究所  
孙秀俊 中国水产科学研究院黄海水产研究所  
赵峰 中国水产科学研究院东海水产研究所  
杨爱国 中国水产科学研究院黄海水产研究所  
刘志鸿 中国水产科学研究院黄海水产研究所  
赵庆 上海海洋大学中国水产科学研究院黄海水产研究所  
张高伟 上海海洋大学中国水产科学研究院黄海水产研究所  
陈夕 上海海洋大学中国水产科学研究院黄海水产研究所  
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中文摘要:
      海洋环境变化及过度捕捞造成生态环境失衡,使得贝类的各种病害问题日渐突显,致病性弧菌引发的病害比较常见。在这种背景下,魁蚶( Scapharca broughtonii)野生资源数量急剧减少,其养殖产业亟待种质改良。为了有效防治细菌及其它病原微生物对魁蚶等贝类的危害,本研究观察了魁蚶各组织中溶菌酶活性对鳗弧菌侵染的响应过程,以期探讨魁蚶体内溶菌酶的免疫功能。本实验采用注射鳗弧菌活菌的方法侵染20月龄魁蚶个体,随机选取16个个体,在每只个体的斧足处注射1ml(约1×109个)鳗弧菌,作为感染组;再随机选取16个个体不注射鳗弧菌作为对照组。两组魁蚶分别于洁净海水中暂养4h,12h,24h,48h后,每组随机取4个魁蚶个体的血液、外套膜、鳃、斧足、肝胰腺、闭壳肌等组织,采用ELISA试剂盒测定其溶菌酶含量变化。实验结果表明,对于鳗弧菌的侵入,魁蚶血液中溶菌酶含量由正常低值迅速增高并一直维持较高的含量,说明血液是魁蚶机体防御病原菌的主要免疫组织之一;魁蚶外套膜在无感染的情况下,对于外界水环境的干扰,始终保持较高的溶菌酶含量;鳃、斧足的溶菌酶含量均在注射细菌24h之后明显高于正常值,说明外套膜、鳃、斧足作为魁蚶机体与外界接触的第一道屏障也能应对病原菌入侵,但反应较血液延迟;肝胰腺和闭壳肌的溶菌酶含量变化不明显,推测肝胰腺和闭壳肌不是魁蚶的重要免疫组织或器官。本研究结果可为魁蚶抗病选育及免疫机理方面的研究提供相关的参数。
英文摘要:
      Changes in the marine environment and the imbalance of ecological environment caused by overfishing have increasingly highlighted various diseases of shellfish, and diseases caused by pathogenic vibrio are relatively common.In this context, the number of wild resources of Scapharca broughtonii has been sharply reduced, and its breeding industry urgently needs germplasm improvement.In order to effectively prevent and control the harm of bacteria and other pathogenic microorganisms on the shellfish such as Scapharca broughtonii, this study observed the response process of the lysozyme activity in the tissues of Scapharca broughtonii to the infection of Vibrio anguillarum, so as to explore the immune function of the lysozyme in the body of Scapharca broughtonii.In this experiment, Vibrio anguillarum was injected into each Scapharca broughtonii individuals. Sixteen individuals were randomly selected, and 1ml (about 1 × 109)Vibrio anguillarum was injected at the axe foot of each individual as the infected group, but not is the control group. The two groups were cultivated in clean seawater for 4h, 12h, 24h and 48h.The blood, mantle, gill, axe foot, hepatopancreas and adductor muscle were randomly selected from 4 individuals in each group. The concentration of lysozyme was determined by ELISA kit. The results showed that the concentration of lysozyme in blood increases rapidly from the normal low value and maintains a high level, indicating that blood is one of the main immune tissues in the organism to defend pathogenic bacteria; .In the case of no infection of the Scapharca broughtonii mantle, the interference of the external water environment always maintained a high lysozyme concentration. The concentration of lysozyme in gills and axe foot are significantly higher than the normal values 24 hours after the injection of bacteria, indicating that the mantle, gills and axe foot, as the first barrier for the body of Scapharca broughtonii to contact with the outside world, could also respond to the invasion of pathogenic bacteria, but the response was delayed compared with the blood. The changes of lysozyme concentration in hepatopancreas and adductor muscles were not obvious, speculating that hepatopancreas and adductor muscles were not important immune tissues or organs of Scapharca broughtonii. The results of this study can provide relevant parameters for the study of disease resistance and selection and immune mechanism of Scapharca broughtonii.
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