文章摘要
四种鲍45S rDNA在染色体上的比较定位
Comparative chromosome mapping of 45S rDNA in four species of abalone
投稿时间:2018-12-20  修订日期:2019-04-01
DOI:
中文关键词:   荧光原位杂交  染色体  45S rDNA
英文关键词: Haliotis  fluorescence in situ hybridization  chromosome  45S rDNA
基金项目:国家基金委促进海峡两岸科技合作联合基金(U1605213)
作者单位E-mail
张健鹏 集美大学水产学院 1573942437@qq.com 
王铜毅 集美大学水产学院  
骆轩 厦门大学海洋与地球学院  
游伟伟 厦门大学海洋与地球学院  
柯才焕 厦门大学海洋与地球学院  
蔡明夷 集美大学水产学院 myicai@jmu.edu.cn 
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中文摘要:
      为了研究皱纹盘鲍、西氏鲍、绿鲍和杂色鲍等4种鲍的核型特征,实验利用荧光原位杂交(fluorescence in situ hybridization, FISH)比较定位了这4种鲍的45S rDNA位点。皱纹盘鲍中,约83%的中期细胞检出2对45S rDNA位点,分别位于13号和16号染色体的长臂端部。西氏鲍中,约75%的中期细胞检出3对45S rDNA位点,分别位于6号染色体短臂端部、14号和17号染色体长臂端部。绿鲍中,约85%的中期细胞检出3对45S rDNA位点,分别位于4号、6号和8号染色体长臂的端部。杂色鲍中,约65%的中期细胞检出3对45S rDNA位点,分别位于3号、4号和12号染色体短臂的端部。此外,4种鲍均有少数中期相的45S rDNA位点数高于众数,这提示除了明确的45S rDNA位点外,4种鲍可能均有若干个不稳定的45S rDNA位点。研究结果丰富了鲍细胞遗传学研究资料,为鲍的遗传育种研究提供了基础数据。
英文摘要:
      Abalone is not only precious seafood in China, but also important mariculture shellfish. In recent years, the genetic breeding research of abalone has made rapid progress, but the research data of cytogenetic analysis is still in lack. Therefore, this study used the fluorescence in situ hybridization (FISH) to compare the distribution of 45S rDNA clusters in Haliotis discus hannai, H. gigantea, H. fulgens, and H. diversicolor. In H. discus hannai, 83% of the metaphase had 2 pairs of 45S rDNA sites, locating at terminal of the long arm ends of chromosomes 13th and 16th, respectively. In H. gigantea, about 75% of the metaphase had 3 pairs of 45S rDNA sites, locating at the terminal of the short arm of chromosome 6th, and at the terminal of the long arm of chromosomes 14th and 17th, respectively. In H. fulgens, about 85% of the metaphase cells detected three pairs of 45S rDNA sites located at the terminal of the long arms of chromosomes 4th, 6th, and 8th. In H. diversicolor, about 65% of the metaphase had 3 pairs of 45S rDNA sites, locating at the terminal of the short arms of chromosomes 3th, 4th, and 12th. In addition to the main mode, there were other low-frequency modes in all the 4 species of abalone, suggesting that these abalone may have several unstable 45S rDNA sites besides the unambiguous sites. The number and the location of 45S rDNA loci in abalone showed a high level of intraspecific variation in general. These findings enrich the cytogenetic research data of abalone and provide basic data for the further studies of genetic breeding in abalone.
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