| Takifugu rubripes were instant killed, bled and gutted, two treatments for 6 days storage are designed including storage in ice and ambient temperature, respectively. During storage, the progression of rigor mortis and body temperature were recorded. Sampling and assay were carried out at both different rigor stages and every storage day. Rigor index, ATP and related compounds, glycogen, pH and phosphoprotein were selected as indicators as postmortem biochemical changes. Main findings were concluded. the onset of rigor mortis for iced treatment start at around 7th hours after slaughtered, and its maximum rigor index was up to 89% with 20-24 hours duration in stiff, the fish body maintained at rigor with rigor index as 9~16% after 4 days icing storage. However, the maximum rigor index of 71% was observed fleetingly in the control treatment with higher temperature under ambient, and the whole rigor progression or stages were undefined. Glycogen degraded sharply at the initial post-mortem period for all treatments. Comparing with the trace amounts was still detected in 5 days storage for the iced treatment, glycogen was almost depleted after 3 days storage in the control treatment. The pH decreasing patterns of two treatments reflected the differences glycogen consumption rates between the two storage treatments. ATP was rapidly depleted in all treatments with accumulation of IMP exclusively. Maximum of IMP reached to 9.5 μmol/g on the 3rd day and remained at about 7.5 μmol/g on the 6th day of the iced treatment. As to the control treatment, IMP was degraded rapidly after the maximum as 9.2 μmol/g on the 1st storage day with higher temperature. Comparing to the iced treatment with a trace Hx amounts, the control treatment present a significant accumulation of Hx. Muscle protein phosphorylation indicated that the rigor mortis progression of Takifugu rubripes was also related to the properties of muscle protein components. Notably, the protein components relating to glycolytic enzymes have a significant difference in phosphorylation between the iced and ambient treatment. The ambient treatment present higher phosphorylation level, which is consistent with the glycolysis properties observed in ambient treatment. In conclusion, Takifugu rubripes had advanced in icing stability and high accumulation of IMP after slaughtered,and the association between protein phosphorylation level and the process of rigor mortis is worth exploring further.