文章摘要
红鳍东方鲀死后僵直及生化变化特性
Postmorten Biochemistry and Rigor Development of Pufferfish (Takifugu rubripes)
投稿时间:2019-01-19  修订日期:2019-04-02
DOI:
中文关键词: 红鳍东方鲀  死后僵直  死后生化特性
英文关键词: Takifugu rubripes  rigor mortis  postmortem biochemical
基金项目:国家重点研发计划资助- 蓝色粮仓科技创新(2018YFD0901001);国家自然科学基金资助项目(31671790);
作者单位E-mail
冷寒冰 大连海洋大学 lenghb001@163.com 
刘俊荣 大连海洋大学 ljunrong@dlou.edu.cn 
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中文摘要:
      以养殖红鳍东方鲀为研究对象,活体速杀放血去脏,设冰藏组和室温组进行6天对照贮藏。贮藏期间观察记录鱼体死后僵直状态并监测僵直指数,跟踪分析肌肉生化变化,生化指标包括ATP及关联物、糖原、pH以及肌肉蛋白磷酸化等。研究结果发现,冰藏组在死后7 h左右才开始发生僵直,僵直指数最大可达89%,并可维持20-24 h;冰藏至第4天,僵直指数仍可分别保持在9%和16%。室温对照组没有明显观察到僵直发生发展过程,虽然测出最大僵直指数为71%,但僵直现象转瞬即逝。死后贮藏过程中糖原迅速下降,室温对照组贮藏至第3天几乎消耗完全,而冰藏组消耗殆尽发生在5天以后;肌肉pH分析结果与糖原呼应,冰藏组的pH值相较于冰鲜组下降较慢,且高于室温对照组。速杀后至僵直开始,各处理组ATP均迅速消耗,IMP快速积累且为主要核苷酸降解产物;冰藏组IMP在第3天左右达到最大值9.5 μmol/g,随后开始呈下降趋势,贮藏到第6天时IMP仍保持在7.5 μmol/g左右,始终处于一个较高水平。室温对照组则IMP在第1天达到最大值9.2 μmol/g后迅速下降,Hx积累量明显增多。蛋白质磷酸化结果表明,鱼体死后僵直状态或程度与肌肉蛋白组分性质也具有一定的关联。值得关注的是与糖酵解酶有关的蛋白组分,其磷酸化程度在室温组和冰藏组之间存在明显差异,即室温组具有更高的磷酸化水平,这与室温组所表现的更加活跃的糖酵解进程相吻合。研究表明,红鳍东方鲀死后有优越的冰藏稳定性和高IMP积累的特点,同时蛋白质磷酸化水平与僵直进程所表现的关联值得进一步探索。
英文摘要:
      Takifugu rubripes were instant killed, bled and gutted, two treatments for 6 days storage are designed including storage in ice and ambient temperature, respectively. During storage, the progression of rigor mortis and body temperature were recorded. Sampling and assay were carried out at both different rigor stages and every storage day. Rigor index, ATP and related compounds, glycogen, pH and phosphoprotein were selected as indicators as postmortem biochemical changes. Main findings were concluded. the onset of rigor mortis for iced treatment start at around 7th hours after slaughtered, and its maximum rigor index was up to 89% with 20-24 hours duration in stiff, the fish body maintained at rigor with rigor index as 9~16% after 4 days icing storage. However, the maximum rigor index of 71% was observed fleetingly in the control treatment with higher temperature under ambient, and the whole rigor progression or stages were undefined. Glycogen degraded sharply at the initial post-mortem period for all treatments. Comparing with the trace amounts was still detected in 5 days storage for the iced treatment, glycogen was almost depleted after 3 days storage in the control treatment. The pH decreasing patterns of two treatments reflected the differences glycogen consumption rates between the two storage treatments. ATP was rapidly depleted in all treatments with accumulation of IMP exclusively. Maximum of IMP reached to 9.5 μmol/g on the 3rd day and remained at about 7.5 μmol/g on the 6th day of the iced treatment. As to the control treatment, IMP was degraded rapidly after the maximum as 9.2 μmol/g on the 1st storage day with higher temperature. Comparing to the iced treatment with a trace Hx amounts, the control treatment present a significant accumulation of Hx. Muscle protein phosphorylation indicated that the rigor mortis progression of Takifugu rubripes was also related to the properties of muscle protein components. Notably, the protein components relating to glycolytic enzymes have a significant difference in phosphorylation between the iced and ambient treatment. The ambient treatment present higher phosphorylation level, which is consistent with the glycolysis properties observed in ambient treatment. In conclusion, Takifugu rubripes had advanced in icing stability and high accumulation of IMP after slaughtered,and the association between protein phosphorylation level and the process of rigor mortis is worth exploring further.
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