文章摘要
中华绒螯蟹二龄早熟和晚熟品系选育第四代在扣蟹阶段养殖性能和免疫性能的评价*
The evaluation of culture and immunity performance of the second-year early-maturing and late-maturing strains of the fourth selective generation during the juvenile culture of Chinese mitten crab (Eriocheir sinensis)
投稿时间:2019-01-25  修订日期:2019-09-05
DOI:
中文关键词: 中华绒螯蟹  扣蟹养殖  免疫性能  攻毒实验  比较研究
英文关键词: Chinese mitten crab  juvenile cultivation  immune performance  pathogen challenge  comparative research
基金项目:农业部现代农业产业技术体系专项资金项目(编号:CARS-48);上海市高水平地方高校建设科研项目(A1-2801-18-1003);上海市科委工程技术中心能力提升项目(16DZ2281200);深圳市澳华农牧有限公司横向项目(D-8006-16-0088)
作者单位E-mail
王海宁 上海海洋大学 农业农村部淡水种质资源重点实验室 1738826774@qq.com 
姜晓东 上海海洋大学 农业农村部淡水种质资源重点实验室  
吴旭干 上海海洋大学 农业农村部淡水种质资源重点实验室  
邓登 浙江澳凌水产种业科技有限公司  
向朝林 浙江澳凌水产种业科技有限公司  
成永旭 上海海洋大学 农业农村部淡水种质资源重点实验室 yxcheng@shou.edu.cn 
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中文摘要:
      本研究以中华绒螯蟹二龄早熟和晚熟群体选育第四代 (G4)的扣蟹为研究对象,并以未经选育的养殖群体扣蟹为对照组,采用养殖实验、免疫指标测定和攻毒实验等方法,从生长性能和免疫抗病性能的角度分析并评价不同群体的蟹种质量。结果显示:(1)养殖实验中两选育群体的最终体重和特定生长率(SGR)均优于对照组,且二龄早熟群体在8-11月的平均体重始终低于晚熟群体,但差异不显著 (P>0.05);(2)就养殖效果而言,三群体成活率和最终产量由大到小依次为早熟G4、晚熟G4、对照组,三群体一龄早熟率的大小次序与之相反,其中雌体产量和一龄早熟率差异显著 (P <0.05); (3)就免疫指标而言,两选育组碱性磷酸酶(AKP)和酸性磷酸酶 (ACP)活性以及总抗氧化能力 (T-AOC)水平均高于对照组,其中雄体肝胰腺中AKP活性以及雌体血淋巴中T-AOC水平差异显著 (P <0.05);(4)两选育群体在攻毒实验中最终死亡率均较低,分别比对照组低29.50%和22.94%。综上,选育G4代扣蟹的生长性能和免疫抗病相关生理指标都表现出了比未选育群体优良的特性,市场应用和产业化前景广阔。
英文摘要:
      The early-maturing (EM) and late-maturing (LM) of second-year E. sinensis have been mass selected to the fourth generation (G4), but their crabseed quality has not been evaluated. Therefore, this study was designed to compare the culture performance, immunity and disease resistance of EM, LM and non-selective crabseed (as the control) with the methods of culture experiment, challenge test and the analysis of non-specific immune indices. The results showed that: (1) The final weight and specific growth rate (SGR) of two selected populations were both relatively higher than the control, and the average weight of EM was lower than that of the LM between August and November, but no significant difference was observed. (2) In terms of culture effect, the order of survival rate and final yield was EM > LM > control with significant difference in yield of females, the order of precocious rate was opposite with significant difference in females either. (3) As for the immune index, the levels of alkaline phosphatase (AKP), acid phosphatase (ACP) and total antioxidant capacity (T-AOC) of two selected populations were higher than the control, with significant difference of ALP level in hepatopancreas of females and significant difference of T-AOC level in hemolymph of males; (4) Cumulative mortalities of two selected populations were both lower (29.50% and 22.94%, respectively) than the control in the challenge test. In conclusion, the crabseed quality of the two selected populations is more favorable, thus crabseed quality improvement of Chinese mitten crab seems feasible.
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