文章摘要
几种经济红藻中茉莉酸合成途径关键物质的研究
Study on the key compounds involve in jasmonic acid biosynthesis pathway in several economic red algae
投稿时间:2019-01-27  修订日期:2019-06-03
DOI:
中文关键词: 茉莉酸合成途径,LC-MS,机械损伤,胁迫响应,红藻
英文关键词: jasmonic acid biosynthesis pathway  LC-MS  mechanical damage  stress response  red algae
基金项目:国家自然科学基金,省自然科学基金
作者单位E-mail
王思琪 宁波大学 413993873@qq.com 
陈海敏 宁波大学 chenhaimin@nbu.edu.cn 
陈娟娟 宁波大学  
杨锐 宁波大学  
骆其君 宁波大学  
吴玮 宁波大学  
崔晓山 宁波大学  
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中文摘要:
      以LC-MS建立茉莉酸生物合成途径相关物质的检测方法,并检测龙须菜、坛紫菜受到机械损伤时各物质的变化。以100%甲醇提取茉莉酸(JA)、茉莉酸甲酯、12-氧-植物二烯酸和13-氢过氧化亚麻酸(13-HpOTE),利用C18色谱柱,以甲醇/水为流动相分离4种物质。优化条件下4种物质得到良好分离,加标回收率为81.23-90.25%,检测限在0.04-0.56 ng/mL,灵敏度高。坛紫菜、龙须菜和真江蓠3种红藻中,坛紫菜未检测到JA和13-HpOTE,另2种藻的4种物质均检测到。龙须菜受机械损伤胁迫后,4种物质在短时间内得到积累,其中13-HpOTE响应迅速。坛紫菜在损伤后检测到JA的产生,但各物质响应速度慢。研究表明,红藻中可能存在类似于植物的茉莉酸合成途径,并参与对损伤的胁迫响应。
英文摘要:
      A quantitative method based on LC-MS was established for detection of compounds involved in the biosynthetic pathway of jasmonic acid and to detect the changes of these compounds in Gracilariopsis lemaneiformis and Pyropia haitanensis when they were subjected to mechanical damage. Jasmonic acid (JA), methyl jasmonate, 12-oxy-phytodienoic acid and 13-hydroperoxylinolenic acid (13-HpOTE) were extracted with 100% methanol, and the four compounds were separated by XBridgeTM C18 chromatographic column with methanol/water as mobile phase. The results showed that the four compounds could be separated well under optimal conditions. The recoveries of the four compounds were 81.23-90.25%, and the detection limits of the method were between 0.04 and 0.56 ng/mL, indicating that the high sensitivity of this method. These four compounds in G. lemaneiformis, Pyropia haitanensis and Gracilaria asiatica were analyzed. In addition to no JA and 13-HpOTE detected in P. haitanensis, four compounds were detected in the other two species of red algae. The content changes of these compounds in G. lemaneiformis under mechanical damage stress were analyzed, and it was found that when the alga was stressed, the contents of them were accumulated within a short time, and the responses of them were rapid. After P. haitanensis was mechanical damaged, JA production was detected, but the response rates of these compounds were slower than G. lemaneiformis. These results suggest that some red algae may have a similar pathway of jasmonic acid synthesis to that of plants, and also may participate in the stress response to mechanical injury.
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