文章摘要
麦穗鱼入侵云南四大水系后基于线粒体Cytb的群体遗传结构分析
Genetic structure analysis of Pseudorasbora Parva in the four major river systems in Yunnan based on mitochondrial Cytb
投稿时间:2019-02-20  修订日期:2019-06-22
DOI:
中文关键词: 遗传多样性  遗传结构  细胞色素 b 基因  麦穗鱼  
英文关键词: Genetic diversity  genetic structure  Cyt b  Pseudorasbora parva
基金项目:国家自然科学基金
作者单位E-mail
杨丽萍 河南师范大学水产学院 weedyung@163.com 
胡俊仪 河南师范大学水产学院  
秦超彬 河南师范大学水产学院  
张玉茹 河南师范大学水产学院  
卢荣华 河南师范大学水产学院  
孟晓林 河南师范大学水产学院  
杨国坤 河南师范大学水产学院  
闫潇 河南师范大学水产学院  
职韶阳 河南师范大学水产学院  
聂国兴 河南师范大学水产学院 niegx@htu.cn 
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中文摘要:
      为揭示麦穗鱼(Pseudorasbora parva) 入侵云南后群体的遗传多样性和遗传分化差异现状,本研究采集了云南澜沧江、怒江、红河、伊洛瓦底江水系13个样点,及黄河、长江、珠江原产地水系5个样点的麦穗鱼群体共计215尾标本,利用线粒体细胞色素b基因(Cyt b)全序列作为分子标记,初步分析了麦穗鱼群体的遗传多样性、遗传结构和遗传分化情况。研究结果共检测到72个变异位点,定义25个Cyt b单倍型。云南四大水系麦穗鱼单倍型多样性和核苷酸多样性分别为0.828±0.014 和0.00544±0.00118。云南四大水系和黄河、长江、珠江水系相比,具有较高的遗传多样性。单倍型系统发育树与单倍型网络图显示,黄河群体单倍型独立,云南各水系单倍型与珠江、长江单倍型混杂,推测云南麦穗鱼主要来源于珠江和长江,这与云南省引种经济鱼类历史一致。云南四大水系麦穗鱼群体间分子变异分析(AMOVA)显示麦穗鱼群体间具有程度较高的遗传分化,其中大多数遗传变异存在于群体内(72.60%), 群体间的遗传变异为28.62%,水系间为1.22%。显示麦穗鱼遗传分化与当前水系的分布格局不吻合。Fu’s Fs 中性检验结果和核苷酸不配对分析结果均表明,云南四大水系麦穗鱼群体没有发生扩张。麦穗鱼进入云南各水系后,单倍型多样性较高,可能来源于多个地区。在后续对麦穗鱼的管理过程中,需要注意避免单倍型特殊的群体与其他地区群体的交流,减少水系间相互引种。此外,开发麦穗鱼资源利用方式,通过促进利用麦穗鱼控制群体数量,以减小其对当地土著物种和渔业养殖的危害。
英文摘要:
      Pseudorasbora Parva is originally distributed in Jinshajiang and Nanpanjiang in Yunnan. However, it has been widely distributed in all major river systems and become an invasive fish in other four river systems in Yunnan. In order to reveal the difference of genetic diversity and genetic differentiation of Pseudorasbora parva between Yunnan after the invasion and the native region, we collected 215 samples distributed in 13 different sites of Lancang River, Nujiang, Honghe and Irrawaddy River in Yunnan, and 5 native populations in Yellow, Yangtze, and Pearl River. Here we analyses a 1072 base pair fragment of the mitochondrial cytochrome b gene to examine the genetic diversity and genetic structure. A total of 72 mutation sites and 25 Cyt b haplotypes were identified. The haplotype diversity and nucleotide diversity of the four river systems in Yunnan were 0.828±0.014 and 0.00544±0.00118, respectively. Haplotype diversity of the introduced populations from the four river systems in Yunnan was higher than that of the native populations. The haplotype maximum likelihood tree and haplotype network map show that the haplotypes of the Yellow River population are special, while haplotype in the invasive populations in Yunnan were mixed with that in Yangtze, and Pearl River. It is speculated that Pseudorasbora parva in Yunnan were mainly came from the Yangtze River and the Pearl River, which was consistent with the history of introduction of economic fish in Yunnan Province. Analysis of molecular variance (AMOVA) revealed significant genetic subdivision among individuals within populations and among populations within rivers but not among rivers, indicating distribution of genetic diversity was inconsistent with contemporary hydrological structure. That may be the human effect who introduced them from many native regions. The results of Fu’s Fs neutral test showed that there was no expansion of the invasive population of Pseudorasbora parva in Yunnan. In the subsequent management of the invasive population of Pseudorasbora parva in Yunnan, it is necessary to avoid the exchange of special haplotype groups with other regional groups, and to reduce the introduction of fish from different river systems. In addition, the development of new ways to utilize Pseudorasbora parva may be a useful way to control their population and reduce the damage to indigenous species and fishery culture in Yunnan.
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