| Hybridization is the most widely used and effective way of artificial breeding in aquaculture. This study use E.lanceolatus as female parent, E. moara as male parent by artificial insemination technology has carried on the interspecific cross experiment, and developmental sequence of fertilized egg, growth of larvae, juvenile and young fish was observed and studied, and then with the parent and the hybrid F1 generation “yunlong grouper” (E.moara ♀× E.lanceolatus) contrasted the phenotypic traits. Results showed that the embryos as oval, transparent, floating, the fertilized eggs size (0.787±0.013)mm, each egg has one oil globule in the center. In sea-water temperature of 24 ℃, salinity 30, pH 7.8, embryos can develop normally, according to the data, six period including 25 stages of embryos were recorded ,which were fertilized egg, cleavage, blastula, gastrula, organogenesis and hatching period, the length of newly hatched larvae（1.595±0.015）mm and shows the feasibility of the cross;3 days after hatching, the yolk membranes disappeared and became the late larva, with a total length of （2.530±0.023）mm. 52 days After hatching, entering the juvenile stage, the total length（17.500±1.915）mm, the length of the second dorsal fin spine (5.795±0.049) mm, and the length of the first pelvic fin (2.992±0.911) mm. When the dorsal fin spine contracted to the minimum value (4.460±0.600) mm, scales began to appear and body color began to darken. The second dorsal fin spine and the first pelvic fin spine completely degenerated. The body shape was similar to that of the adult fish. The whole body was covered with scales. The growth comparison results showed that the growth rate of the positive and negative cross F1 generation was between that of the parents, which was faster than that of the E. moara, which was slower than that of the E. lanceolatus, and the growth rate of the reverse cross F1 generation was slower than that of the orthogonal F1 generation. The results provide a basis for the formation of hybrid dominant traits and genetic analysis of grouper.