文章摘要
鞍带石斑鱼(♀)×云纹石斑鱼(♂)杂交后代早期发育及正反杂交后代生长特性研究
Early development of hybrids of Epinephelus lanceolatus♀×Epinephelus moara♂ and growth characteristics of reciprocal crosses
投稿时间:2019-03-06  修订日期:2019-06-03
DOI:
中文关键词: 鞍带石斑鱼  云纹石斑鱼  云龙石斑鱼  正交  反交  胚胎发育  变态发育
英文关键词: Epinephelus.lanceolatus, E. moara, Yunlong grouper  ,Embryos,Abnormal development, Orthogonal offspring
基金项目:山东省重点研发计划(2019),中国水产科学研究院黄海水产研究所基本科研业务费(20603022019002,20603022017013),烟台市科技发展计划(2016JH021)和烟台市“双百计划”人才项目
作者单位E-mail
李子奇 农业部海洋渔业可持续发展重点实验室 中国水产科学研究院黄海水产研究所 青岛 lzqshou@163.com 
成美玲 农业部海洋渔业可持续发展重点实验室 中国水产科学研究院黄海水产研究所 青岛  
吴玉萍 农业部海洋渔业可持续发展重点实验室 中国水产科学研究院黄海水产研究所 青岛  
张晶晶 农业部海洋渔业可持续发展重点实验室 中国水产科学研究院黄海水产研究所 青岛  
李振通 农业部海洋渔业可持续发展重点实验室 中国水产科学研究院黄海水产研究所 青岛  
赵谭军 农业部海洋渔业可持续发展重点实验室 中国水产科学研究院黄海水产研究所 青岛  
马文辉 莱州明波水产有限公司 烟台  
庞尊方 莱州明波水产有限公司 烟台  
翟介明 莱州明波水产有限公司 烟台  
李波 莱州明波水产有限公司 烟台  
田永胜 农业部海洋渔业可持续发展重点实验室 中国水产科学研究院黄海水产研究所 青岛 tianys@ysfri.ac.cn 
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中文摘要:
      以鞍带石斑鱼为母本,云纹石斑鱼为父本,采用人工授精技术进行了种间反交实验,并对反交子代的胚胎发育和仔稚幼鱼的生长情况进行了观察,进而与亲本及正交F1云龙石斑鱼(云纹石斑鱼(Epinephelus moara)♀×鞍带石斑鱼(Epinephelus lanceolatus)♂)的表型性状进行对比分析。结果显示,反交子代受精卵为圆形、透明、浮性卵,卵径为(0.787±0.013)mm,有1个油球,居卵中央,在水温为24℃、盐度30、pH为7.8的孵化条件下,胚胎可以正常发育,经历受精卵、卵裂、囊胚、原肠胚、器官形成和出膜6个阶段25个时期完成胚胎发育,初孵仔鱼全长为(1.595±0.015)mm,说明反交的可行性。孵化后3 d,卵黄囊消失,由前期仔鱼发育为后期仔鱼,全长(2.530±0.023)mm。孵化后52 d,进入稚鱼期,全长(17.500±1.915)mm,第二背鳍棘长(5.795±0.049)mm,第一腹鳍棘长(2.992±0.911)mm;58 d稚鱼背鳍棘收缩至最小值时(4.460±0.600)mm,鳞片和体色开始出现。孵化后67 d,进入幼鱼期,全长(34.500±1.291)mm,第二背鳍棘和第一腹鳍棘完全退化,体型与成鱼相似,全身覆盖鳞片,反交F1体色不一致,具4种表现型。生长对比结果显示,正反交F1的发育速率均介于亲本之间,均快于云纹石斑鱼,慢于鞍带石斑鱼,反交F1生长速率慢于正交F1。研究结果为石斑鱼杂交优势性状形成及遗传解析提供了一定数据基础。
英文摘要:
      Hybridization is the most widely used and effective way of artificial breeding in aquaculture. This study use E.lanceolatus as female parent, E. moara as male parent by artificial insemination technology has carried on the interspecific cross experiment, and developmental sequence of fertilized egg, growth of larvae, juvenile and young fish was observed and studied, and then with the parent and the hybrid F1 generation “yunlong grouper” (E.moara ♀× E.lanceolatus) contrasted the phenotypic traits. Results showed that the embryos as oval, transparent, floating, the fertilized eggs size (0.787±0.013)mm, each egg has one oil globule in the center. In sea-water temperature of 24 ℃, salinity 30, pH 7.8, embryos can develop normally, according to the data, six period including 25 stages of embryos were recorded ,which were fertilized egg, cleavage, blastula, gastrula, organogenesis and hatching period, the length of newly hatched larvae(1.595±0.015)mm and shows the feasibility of the cross;3 days after hatching, the yolk membranes disappeared and became the late larva, with a total length of (2.530±0.023)mm. 52 days After hatching, entering the juvenile stage, the total length(17.500±1.915)mm, the length of the second dorsal fin spine (5.795±0.049) mm, and the length of the first pelvic fin (2.992±0.911) mm. When the dorsal fin spine contracted to the minimum value (4.460±0.600) mm, scales began to appear and body color began to darken. The second dorsal fin spine and the first pelvic fin spine completely degenerated. The body shape was similar to that of the adult fish. The whole body was covered with scales. The growth comparison results showed that the growth rate of the positive and negative cross F1 generation was between that of the parents, which was faster than that of the E. moara, which was slower than that of the E. lanceolatus, and the growth rate of the reverse cross F1 generation was slower than that of the orthogonal F1 generation. The results provide a basis for the formation of hybrid dominant traits and genetic analysis of grouper.
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