文章摘要
基于高通量测序开发的熊本牡蛎(Crassostrea sikamea)微卫星标记评价野生、养殖群体的遗传多样性及通用性分析
Evaluation of genetic diversity and versatility of wild and cultured populations based on high-throughput sequencing of Crassostrea sikamea microsatellite markers
投稿时间:2019-03-09  修订日期:2019-06-06
DOI:
中文关键词: 熊本牡蛎  微卫星标记  野生群体  养殖群体  通用性
英文关键词: Crassostrea sikamea  microsatellite markers  wild population  cultured population  transferability
基金项目:市自然科学基金,省自然科学基金,国家自然科学基金
作者单位E-mail
黄飘逸 重庆师范大学 生命科学学院 重庆 17725027556@163.com 
马海涛 中国科学院南海海洋研究所 广州  
喻子牛 中国科学院南海海洋研究所 广州  
张跃环 中国科学院南海海洋研究所 广州  
高红梅 重庆师范大学 生命科学学院 重庆  
彭建军 重庆师范大学 生命科学学院 重庆  
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中文摘要:
      利用高通量测序的方法,从熊本牡蛎基因组中开发出20对具有多态性的微卫星引物。用一个野生群体对所有微卫星引物进行了评价,共扩增出330个等位基因,等位基因数范围为6~39,平均等位基因数为16.5;有效等位基因数(Ne)范围为1.3529~33.3617;观测杂合度(Ho)范围为0.2000~1.0000;期望杂合度(He)范围为0.2656~0.9877;香农-威纳指数(I)范围为0.6483~3.5858;多态信息含量(PIC)范围为0.2545~0.9692,共有16个位点符合Hardy-Weinberg平衡。利用这些位点对一个养殖群体进行了遗传多样性分析,发现平均等位基因数为10.25;平均有效等位基因数(Ne)为5.8434。观测杂合度(Ho)平均值为0.6391;期望杂合度(He)平均值为0.7636;Shannon-wiener指数(I)平均值为1.7914;多态信息含量(PIC)平均值为0.7207。发现熊本牡蛎养殖群体的遗传多样性低于野生群体,但仍然维持在高度多态水平。本研究结果说明在熊本牡蛎人工繁育过程中,使用大数量的亲本进行繁育,可有效防止选育群体的遗传多样性降低,但人工选育对选育群体的遗传多样性也产生了一定的影响。另外分析了这些引物在近缘种葡萄牙牡蛎、太平洋牡蛎、香港牡蛎、有明牡蛎、僧帽牡蛎、咬齿牡蛎以及舌骨牡蛎中的通用性情况,发现XB1-6、XB1-39、XB1-45三个位点在8个物种中均能扩增出目的条带,XB1-41仅能在熊本牡蛎中扩增出目的条带。
英文摘要:
      Using high-throughput sequencing,we characterized 20 pairs of polymorphic microsatellite primers from the Crassostrea sikamea genome.,and we examined the markers in a wild population. A total of 330 alleles were found in 25 microsatellites.The observed number of alleles (Na) ranged 6—39 in average of 16.5,and the effective number of alleles(Ne) ranged 1.3529~33.3617. The observed and expected heterozygosity values range 0.2000~1.0000 and 0.2656~0.9877, respectively.The Shannon Wiener index ranged 0.6483~3.5858 and Polymorphic information content (PIC) ranged 0.2545~0.9692. 16 microsatellite markers accord with Hardy-Weinberg equilibrium (HWE). Using these loci to analyze the genetic diversity of a cultured population, the average number of alleles was 10.25; the average number of effective alleles (Ne) was 5.8434. The observed heterozygosity (Ho) average was 0.6391; the expected heterozygosity (He) average was 0.7636; the Shannon-wiener index (I) average was 1.7914; and the polymorphic information content (PIC) average was 0.7207. It was found that the genetic diversity of the C.sikamea cultured population was lower than that of the wild population, but it maintained a high-genetic diversity. The results of this study indicate that in the artificial breeding process of C.sikamea, the use of a large number of parents for breeding can effectively prevent the genetic diversity of the cultured population, but artificial breeding also has a certain impact on the genetic diversity of the cultured population. The 20 loci were surveyed the cross-species proportions in Crassostrea angulate,Crassostrea gigas,Crassostrea hongkongensis,Crassostrea ariakensis,Saccostrea cucullata,Ostrea mordax,Hyotissa hyotis. Locis XB1-6, XB1-39, and XB1-45 can amplify the target band in 8 species, and the loci XB1-41 can only amplify the target band in C.sikamea.
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