文章摘要
两个条斑紫菜耐高温品系的耐低盐特性
Low-salinity tolerance of two high-temperature resistant strains in Pyropia yezoensis
投稿时间:2019-03-12  修订日期:2019-05-15
DOI:
中文关键词: 条斑紫菜  叶状体  叶绿素荧光  耐高温  耐低盐  逆境胁迫
英文关键词: Pyropia yezoensis  blade  chlorophyll fluorescence  high-temperature resistance  low-salinity tolerance  abiotic stress
基金项目:国家自然科学基金,国家重点基础研究发展计划,其它
作者单位E-mail
黄林彬 上海海洋大学 lbhuang@shou.edu.cn 
黄文 宁德海洋环境监测中心站  
严兴洪 上海海洋大学 xhyan@shou.edu.cn 
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中文摘要:
      以两个条斑紫菜耐高温品系(YZ-4和TM-18)为对象,以野生型品系(WT)做对照,分析其叶状体在高温、低盐胁迫下的生长和光系统Ⅱ最大光量子产量(Fv/Fm)的数值,为筛选既耐高温又耐低盐的条斑紫菜抗逆品系提供依据。结果显示,在适宜培养条件(温度18℃和盐度26)下,YZ-4和TM-18叶状体在50~85 d的生长快于WT,Fv/Fm和主要光合色素含量均高于WT,各品系的绝对生长率和Fv/Fm之间均具有较高的正相关性(r≥0.918)。另外,WT叶状体在70 d左右开始形成精子囊时,绝对生长率和Fv/Fm明显下降。在高温胁迫下培养,各品系叶状体的Fv/Fm和绝对生长率均呈下降趋势,且胁迫的温度越高或时间越长,其降幅越大。在24℃高温胁迫35 d后,WT、YZ-4和TM-18的Fv/Fm分别下降了56.7%、43.2%和28.7%。在25℃高温胁迫15 d时,WT的生长完全停滞,而YZ-4和TM-18则分别保持0.51和0.84 cm/d的绝对生长率,说明二者确实是耐高温的。低盐胁迫对叶状体的影响与高温胁迫的类似,在盐度为9的低盐下培养35 d后,WT、YZ-4和TM-18的Fv/Fm降幅分别是46.2%、42.0%和32.0%,三者的绝对生长率分别是0.12、0.10和0.90 cm/d。另外,WT和YZ-4在盐度5时生长受到明显影响,叶状体在15 d时颜色加深、卷曲变硬并腐烂,而TM-18在相同盐度下培养25 d也未腐烂,Fv/Fm仍高达初始值的72.8%。这表明TM-18在高温或低盐胁迫下的耐受性比WT和YZ-4的都强,其内在原因是叶状体Fv/Fm的降幅较小,间接说明Fv/Fm可作为条斑紫菜抗逆品系选育的新指标。
英文摘要:
      The growth and maximum photon quantum yield of photosystem II (Fv/Fm) of the blades of two high-temperature resistant strains (YZ-4 and TM-18) and one wild-type strain (WT, used as control group) in Pyropia yezoensis were analyzed under high-temperatures or low-salinities, in order to screen out strains resistant to the two abiotic stresses. The results showed that YZ-4 and TM-18 blades grew faster than those of WT between 50-85 d under the optimal culture condition (18oC and salinity of 26). The ratios of Fv/Fm and contents of photosynthetic pigments were also higher than those of WT. The absolute growth rate and Fv/Fm of every strain showed high positive correlations (r≥0.918). In addition, WT blades began to form spermatangia around 70 d, their absolute growth rate and Fv/Fm decreased significantly. When cultured under high-temperatures, Fv/Fm and absolute growth rates of the blades of every strain decreased. The higher the temperature or the longer the stress lasted, the greater the decline would be. Fv/Fm of WT, YZ-4 and TM-18 decreased by 56.7%, 43.2% and 28.7%, respectively, after 35 d of high-temperature stress at 24oC. The growth of WT was completely stagnated after 15 d of high-temperature stress at 25oC. Meantime, growth rates of YZ-4 and TM-18 were 0.51 and 0.84 cm/d, respectively, indicating that they were veritable high-temperature resistant strains. The effects of low-salinity stress on the blades were similar to that of high-temperature stress. After 35 d of low-salinity stress at salinity of 9, Fv/Fm of WT, YZ-4 and TM-18 decreased by 46.2%, 42.0% and 32.0%, respectively. The absolute growth rates of WT, YZ-4 and TM-18 were 0.12, 0.10 and 0.90 cm/d, respectively. Besides, growth of the blades of WT and YZ-4 cultured under salinity of 5 was greatly affected, in that the blades were deepened in color, curled and rotted after 15 d under stress, while TM-18 did not rot at the same salinity of 5 for 25 d. These results indicated that TM-18 was more tolerant to high-temperature and low-salinity stress than WT and YZ-4, the internal reason was that the decrease of Fv/Fm of TM-18 blades was small. It was indicated indirectly that Fv/Fm could be used as a new index for breeding of stress-resistant strains in P. yezoensis.
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