| The emergence of novel variety “All-male NO.1” dramatically promoted the development of the yellow catfish industry, of which the cultivation of YY super-male was a critical step. Recently, YY super-male yellow catfish with intersexual gonad emerged largely, which hindered the development of yellow catfish industry. We only found the problem of gonad development in YY super-male breeding with tubificidae by checking the production and breeding records for nearly 10 years. Therefore, four different baits including Artemia salina, Zooplankton, Chironomus plumosus, Tubificidae were used to treat YY super-male for 20 days (11 to 30 days post hatching). The survival rate, body length, and body weight of each group were measured at 60 dph (day post-hatching). Tubificidae treatments significantly increased the body length and weight compared with other baits, and the survival rate of fish fed with Artemia salina was significantly lower than fish fed with the other three baits. In addition, we performed histology analysis on gonadal structure at 60 dph and 1 year old and statistics of fertilization rate at 1 year old. As a result, the fish group fed with Tubificidae displayed 75% intersexual gonad and 25% testis without Seminiferous lobule, and the fertilization rate was only 36.70±4.05%, which was significantly lower than the other groups. In order to study the reasons for the feminization of YY yellow catfish, we measured the estradiol content and found that the estradiol content was low in all four different animal baits. It is speculated that the feminization of YY super-male yellow catfish may be caused by the environmental endocrine disruptors (EDCS) enriched by Tubificidae. Therefore, zooplankton or Chironomus plumosus could be fed in the early stage of large-scale breeding of YY-supermale yellow catfish, whereas Tubificidae should not be fed.