文章摘要
基于新开发微卫星标记评价两个番红砗磲(Tridacna crocea)野生群体的遗传多样性及近缘种通用性检测
MICROSATELLITE MARKERS BASED ON NEW DEVELOPMENT TO EVALUTION THE GENETIC DIVERSITY OF TWO WILD POPULATIONS OF Tridacna crocea AND TRANSFERABILITY TO RELATED SPECIES
投稿时间:2019-03-22  修订日期:2019-05-26
DOI:
中文关键词: 番红砗磲  微卫星标记  野生群体  遗传多样性  通用性
英文关键词: Tridacna crocea  microsatellites  wild population  genetic diversity  transferability
基金项目:市自然科学基金,国家自然科学基金
作者单位E-mail
高红梅 重庆师范大学 177250249501@163.com 
马海涛 中国科学院南海海洋研究所  
喻子牛 中国科学院南海海洋研究所  
张跃环 中国科学院南海海洋研究所  
肖述 中国科学院南海海洋研究所  
黄飘逸 重庆师范大学  
彭建军 重庆师范大学 jjpeng74@163.com 
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中文摘要:
      采用磁珠富集和PCR筛选相结合的方法,得到19个番红砗磲(Tridacna crocea)的多态性微卫星标记。利用新开发微卫星标记对西沙两个野生群体的遗传多样性进行了比较分析,七连屿海域野生群体和永兴岛海域野生群体的平均观测等位基因数(Na)分别为11.1053、11.8947,平均有效等位基因数(Ne)分别为6.2737、6.1727,平均期望杂合度(He)分别为0.7764、0.7880,平均多态信息含量分别为0.7304、0.7439,发现两个野生群体的遗传多样性都处于高度多态水平,说明其有效群体大小仍然保持在较高水平,具有较大的选育潜力。Bonferroni校正后在两个群体中各有4个位点偏离Hardy-Weinberg平衡。另外分析了这些引物在近缘种中的通用性情况,发现在鳞砗磲中有7对通用,6对具有多态性;无鳞砗磲3对通用,1对具有多态性;诺瓦砗磲5对通用,5对具有多态性;长砗磲9对通用,8对具有多态性;砗蚝2对通用,2对具有多态性。
英文摘要:
      We obtained 19 pairs of microsatellite primers from Tridacna crocea by using magnetic-bead enrichment and PCR screening method. Comparison between two wild population in xisha Islands with these markers shows that: the mean allele number and the effective allele number were 11.1053, 11.8947and 6.2737, 6.1727 respectively; the values of average expected heterozygosity were 0.7764 and 0.7880; the mean PIC was 0.7304 and 0.7439; High-genetic diversity was maintained in those wild population. Four loci significantly deviated from Hardy-Weinberg equilibrium after Bonferroni correction in each population. It indicated that the effective population size remained at a high level and had great breeding potential. In addition, crosss-species priming was tested in five additional Tridacna species, the test resulted in 7 loci a- mplifying and 6 loci polymorphisming in T.squamos;3 loci amplifying and 1 loci polymorphism -ing in T.derasa;5 loci amplifying and 5 loci polymorphisming in T.noae;9 loci amplifying and 8 loci polymorphisming in T.maxima;2 loci amplifying and 2 loci polymorphisming in H.hippopus.
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