文章摘要
哈尼梯田稻-鲤综合种养模式下微生物群落结构研究
Studies on microbial community structure of the rice-fish co-culture systems in the Hani Terraces
投稿时间:2019-04-03  修订日期:2019-07-09
DOI:
中文关键词: 哈尼梯田  稻-鲤综合种养  菌群结构  高通量测序
英文关键词: Hani Terraces  the rice-fish co-culture systems  microbial community structure  illumina high-throughput sequencing.
基金项目:
作者单位E-mail
聂志娟 中国水产科学研究院淡水渔业研究中心 niezj@ffrc.cn 
李非凡 中国科学院大学  
赵文武 全国水产技术推广总站  
徐钢春 中国水产科学研究院淡水渔业研究中心  
刘波 中国水产科学研究院淡水渔业研究中心  
王裕玉 中国水产科学研究院淡水渔业研究中心  
邵乃麟 中国水产科学研究院淡水渔业研究中心  
胡佳雯 中国水产科学研究院淡水渔业研究中心  
徐跑 中国水产科学研究院淡水渔业研究中心  
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中文摘要:
      为了探究哈尼梯田稻渔综合种养模式(复合生态系统)微生物菌落结构特征,本实验拟采用高通量测序技术对稻-鲤共作(RC)与稻田单作(CK)两种模式下水体、表层以及根系土壤及鲤鱼肠道细菌(16S rDNA)多样性特征进行分析。高通量测序结果显示:复合生态系统中主要细菌门类为变形菌门、放线菌门、厚壁菌门、绿弯菌门、酸杆菌门、拟杆菌门、硝化螺旋菌门、拟杆菌门等。与CK相比,RC表层土壤中绿弯菌门和厚壁菌门相对丰度显著增加,主要显著增加的优势菌属为厌氧绳菌(Anaerolineaceae_norank)、Aminicenantes_p_norank 、未定义的绿弯菌(Chloroflexi _P_unclassified)、梭菌(Clostridium_sensu_stricto_1)、梭菌(Clostridium_sensu_stricto_12)、互营菌(Syntrophaceae_f_norank)、芽单胞菌(Gemmatimonadaceae_f_norank);RC根系土壤中绿弯菌门显著高于水稻单作组,主要是Anaerolineaceae_norank 、Sideroxydans 、Aminicenantes_p_norank、Chloroflexi _p_unclassified、Gemmatimonadaceae_f_norank优势菌属显著增加。稻-鲤综合种养模式中鲤鱼肠道优势菌主要为厚壁菌门和变形菌门,其中厚壁菌门相对丰度为58.59%,超过一半的丰度。稻-鲤共作模式组相比稻田单作组,土壤的有机质、总氮、总磷等理化指标都有所降低,其中PH 、总磷和速效磷显著降低;稻田表层和根系土壤的细菌Sobs指数及PD指数也均增加,其中稻田表层土壤组显著增加。结果分析显示,增加鲤鱼养殖后,稻田表层土壤的理化性质和细菌群落结构产生明显变化,同时也在一定程度上对水稻根系和养殖生物肠道的菌群产生了影响。研究初步揭示了稻-鲤共作复合生态系统优于稻田单作系统的微生物生态基础。
英文摘要:
      This study was conducted to explore the ecological advantages that occured in the rice-fish co-culture systems in Hani Terrace. High-throughput sequencing technique was used to analysis the bacterial diversity of water, surface soil, root soil and carp intestine (16s rDNA) between rice-carp co-culture (RC) and rice-monoculture (CK) models. The results shown that proteobacteria, actinomycetes, firmicutes, chlorobacteria, acidobacteria, bacteroidetes, nitrospira, bacteroidetes were the dominant phyla the rice-fish co-culture systems. Compared with CK group, the relative abundance of chlorobacteria and firmicutes in RC surface soil significantly increased, and the significantly increased genera mainly including Anaerolineaceae_norank, Aminicenantes_p_norank, Chloroflexi _P_unclassified, Clostridium_sensu_stricto_1, Clostridium_sensu_stricto_12, Syntrophaceae_f_norank, Gemmatimonadaceae_f_norank. The relative abundance of Chlorobacteria in root soil was significantly increased in RC group compared with CK group, and the significantly increased genera including Anaerolineaceae_norank, Sideroxydans, Aminicenantes_p_norank, Chloroflexi _p_unclassified, Gemmatimonadaceae_f_ norank. The dominant phyla of intestine bacterial communities in RC group were firmicutes and proteobacteria, and the relative abundance of firmicutes was 58.59%. The PH, total nitrogen, ammonia-N, nitrate-N, hydrolysis nitrogen, total phosphorus, available phosphorus and soil organic carbon contents were lower in the surface soil of RC group than the CK group, and the PH, total P and available P were significantly reduced. Sobs and Shannon indexes of bacteria in surface and root soil of RC group were also increased, and these two indexes were significantly increased in the surface soil of RC group. In brief, the introduction of fish to rice farming ecosystems changed the physico-chemical properties and bacterial community structure of surface soils, and also affected the microbiota community structure of root soil and fish intestine to a certain extent. Our preliminary results provides microbial ecological evidences for the advantages of rice-carp co-culture over rice monoculture system.
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