文章摘要
水体中泼洒复合乳杆菌对罗非鱼养殖池塘环境、肠道和鳃健康的影响
Effect of mixed culture of Lactobacillus as water additive on the environment of pond, and the health of intestine and gill of tilapia
投稿时间:2019-04-11  修订日期:2019-08-08
DOI:
中文关键词: 罗非鱼  干酪乳杆菌  酸鱼乳杆菌  水质  免疫力  抗氧化能力  菌群结构
英文关键词: tilapia  Lactobacillus casei  Lactobacillus acidophilus  water quality  immunity capacity  antioxidant capacity  microbiota structure
基金项目:现代农业产业技术体系专项资金;广东省促进经济发展专项资金
作者单位E-mail
王淼 中国水产科学研究院珠江水产研究所 miaowfly@163.com 
卢迈新 中国水产科学研究院珠江水产研究所 mx-lu@163.com 
衣萌萌 中国水产科学研究院珠江水产研究所  
李忠徽 中国水产科学研究院珠江水产研究所  
高风英 中国水产科学研究院珠江水产研究所  
刘志刚 中国水产科学研究院珠江水产研究所  
可小丽 中国水产科学研究院珠江水产研究所  
曹建萌 中国水产科学研究院珠江水产研究所  
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中文摘要:
      研究在罗非鱼养殖池塘中添加复合乳杆菌对养殖池塘环境、罗非鱼肠道和鳃健康的影响,为乳杆菌在罗非鱼健康养殖中的应用提供研究基础。在尼罗罗非鱼(Oreochromis niloticus)养殖池塘水体中泼洒复合乳杆菌(对照组:0;实验组:1.0×104 cfu/mL)。复合乳杆菌的成分为干酪乳杆菌(Lactobacillus casei)和酸鱼乳杆菌(L. acidipiscis),比例为1:2.2。监测水质指标、罗非鱼肠道和鳃的免疫及抗氧化指标,并运用高通量测序技术(Illumina MiSeq),检测对照组和实验组水体、底泥、罗非鱼肠道和鳃粘膜共生菌群落结构差异。实验结果表明,从第7周开始,实验组水体中的硝酸盐含量始终低于对照组,实验组比对照组降低了72.68%、72.00%、26.27%和21.46%;实验组水体中的总氮含量在从第6周开始低于对照组,分别降低了43.39%、44.64%、15.64%、37.57%和34.49%;实验组水样中的总磷含量从第4周开始低于对照组,分别降低了40.91%、33.33%、50.00%、33.33%、17.39%、39.29%和25.71%。与对照组相比,实验组罗非鱼肠道的碱性磷酸酶(AKP)活性提高了45.04%,实验组罗非鱼鳃的总超氧化物歧化酶(SOD)和AKP分别提高了58.26%和60.99%。复合乳杆菌的添加对罗非鱼养殖池塘水体菌群结构影响较大,并未影响池塘底泥的菌群结构,影响了罗非鱼肠道和鳃的菌群结构,其中条件致病菌不动杆菌属(Acinetobacter)、泛菌属(Pantoea)细菌的相对丰度降低,而有益细菌鲸杆菌属(Cetobacterium)的相对丰度增加。因此,在养殖水体中泼洒适当浓度的复合乳杆菌可以改善池塘水质,提高罗非鱼肠道和鳃的免疫力、抗氧化能力,同时调节养殖水体、罗非鱼肠道和鳃的菌群结构。
英文摘要:
      To provide a basis for the application of Lactobacillus in the healthy aquaculture of tilapia, the effect of mixed culture of Lactobacillus as water additive on the environment of pond, and the health of intestine and gill of tilapia were studied. Mixed culture of Lactobacillus were added to the water of tilapia (Oreochromis niloticus) pond (control group: 0; experimental group: with a final concentration of 1.0×104cfu/mL). The components of mixed culture of Lactobacillus were Lactobacillus casei and Lactobacillus acidophilus, with a 1:2.2 ratio. The water quality, immune and antioxidant indexes of the intestine and gill of tilapia were monitored. High throughput sequencing technique (Illumina Miq) was used to compare the community structure of microbiota in water, sediment, intestine and gill mucosa of tilapia between the control and the experimental group. The results showed that the nitrate content in the water samples of the experimental group was lower than that of the control group. From the seventh week, and the nitrate content in the water samples of the experimental group was 72.68%, 72.00%, 26.27% and 21.46% lower than that of the control group, respectively. The total nitrogen content in the water samples of the experimental group was 43.39%, 44.64%, 15.64%, 37.57% and 34.49% lower than that of the control group from the sixth week, respectively. The total phosphorus content in the water samples of the experimental group was 40.91%, 33.33%, 50.00%, 33.33%, 17.39%, 39.29% and 25.71% lower than that of the control group from the fourth week, respectively. Compared with the control group, the activity of alkaline phosphatase (AKP) in the intestine of tilapia in the experimental group increased by 45.04%, and the total superoxide dismutase (SOD) and AKP in the gill of tilapia in the experimental group increased by 58.26% and 60.99%, respectively. Mixed culture of Lactobacillus influenced the community structure of microbiota of pond water, but did not affect that of sediment. Microbiota of intestine and gill of tilapia were affected by the probiotic, among which, the conditional pathogens, Acinetobacter and Pantoea were decreased, while the beneficial bacteria, Cetobacterium was increased. Using mixed culture of Lactobacillus as water additive could improve water quality, the immunity and antioxidant capacity of intestine and gill of tilapia, and regulate the community structure of microbiota of intestine and gill of tilapia.
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