文章摘要
抑制气呼吸导致大鳞副泥鳅 (Paramisgurnus dabryanus) 主要呼吸器官的组织病理变化观察
The pathological changes of the major respiratory organs of Paramisgurnus dabryanus with the inhibition of aerial respiration
投稿时间:2019-04-15  修订日期:2019-05-04
DOI:
中文关键词: 大鳞副泥鳅  气呼吸  抑制  呼吸器官  组织病理
英文关键词: Paramisgurnus dabryanus  Aerial respiration  Inhibition  Respiratory organs  Histopathology
基金项目:国家自然科学基金
作者单位E-mail
林蠡 仲恺农业工程学院 linli@zhku.edu.cn 
王玟秀 仲恺农业工程学院  
秦真东 仲恺农业工程学院  
Sarath Bubu V 仲恺农业工程学院  
周萌 仲恺农业工程学院  
梁日深 仲恺农业工程学院  
莫金凤 仲恺农业工程学院  
吴灶和 仲恺农业工程学院  
张轩 广州市华轩水产有限公司  
赵丽娟 仲恺农业工程学院  
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中文摘要:
      大鳞副泥鳅(Paramisgurnus dabryanus)是我国重要的淡水养殖鱼类,其除了用鳃进行呼吸外,也可进行气呼吸。目前关于气呼吸对泥鳅的生理作用还未阐明。本文拟通过抑制大鳞副泥鳅气呼吸,研究其主要呼吸器官的组织病理变化。结果表明被抑制气呼吸的大鳞副泥鳅通常在一周左右出现死亡。当被抑制气呼吸的大鳞副泥鳅出现垂死时,采集对照组和实验组的鳃、皮肤、前肠、中肠、后肠和直肠进行苏木素-伊红(H&E)染色、阿利新蓝-高碘酸雪夫氏(AB-PAS)染色和扫描电镜观察,主要的病理变化包括:(1)H&E染色结果显示实验组泥鳅鰓丝末端充血,背部皮肤表皮层的毛细血管收缩并减少,真皮层细胞呈畸形,前肠黏膜褶膨大,后肠、直肠浆膜层有血红细胞渗出,结缔组织显著增厚;(2)AB-PAS染色结果显示实验组泥鳅鳃、背部皮肤、后肠、直肠组织均出现嗜酸性空泡细胞增多,前肠和中肠黏膜下层酸性黏蛋白含量增多,固有层中性黏蛋白减少;(3)扫描电镜结果显示实验组泥鳅鳃小片表面呈皱褶,表皮受损脱落,背部皮肤表面分泌孔增加,中肠内腔表面突起增多,后肠和直肠絮状颗粒增多。综上所述,抑制大鳞副泥鳅气呼吸会引发其主要呼吸器官上皮组织出现黏液细胞增多、血红细胞溢出等病理变化,甚至导致死亡,气呼吸对大鳞副泥鳅维持正常生理活动是必须的。本研究将为大鳞副泥鳅的健康养殖及幼苗培育提供新的参考。
英文摘要:
      Paramisgurnus dabryanus is an important freshwater aquaculture fish in China. It can also perform aerial respiration in addition to breathing with gill. However, the physiological effects of aerial inhibition on loach have not yet been elucidated. In this study, the histopathology of the main respiratory organs of the P. dabryanus was studied by inhibiting aerial respiration. The results showed that the inhibition of aerial respiration could cause the death of P. dabryanus around one week post of the inhibition. When the fish from the experimental group were moribund, the gills, dorsal skin, anterior intestine, mid intestine, posterior intestine, and rectum were harvested and subjected to Hematoxylin-eosin (H&E) staining, Alcian-Blue Periodic Acid-Schiff (AB-PAS) staining and scanning electron microscopy (SEM). The results showed that: (1). By H&E staining, the gill terminal of the fish from the experimental group was congested, the blood capillary in the epidermis of dorsal skin were contracted and decreased, and dermis cells were deformed. The plicamucosa of anterior intestine was enlarged; meanwhile the connective tissues were denser in the rectum as well as with the exudation of red blood cells (RBc) in the serosa of posterior intestine. (2) By AB-PAS staining, the gills, dorsal skins, posterior intestine, and rectum of the fish from the experimental group revealed an increase in acidophilic vacuoles. Besides, the amount of acid mucoprotein on the submucosa layer of anterior and middle intestines were higher in the experimental group. By contrast, the neutrophil mucoprotein in the lamina propria was decreased in the experimental group. (3) By SEM, the surface of the gill lamellae was damaged and detached, resulted in wrinkling, together with the increased number of secretory pores on the surface of dorsal skin. More bumps were observed on the inner surface of middle intestine of fish from the experimental group. In addition, the granules on the inner surface of the posterior intestine and the rectum of the fish from the experimental group were increased. Taken together, inhibition of aerial respiration could cause an increase of the mucous cells and exudation of RBcs in the main respiratory organs, eventually it could lead to death. In summary, aerial respiration is essential for maintaining the normal physiological features of P. dabryanus. The current results will shed a new light on the seeding cultivation and sustainable culture of P. dabryanus.
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