文章摘要
泥蚶生长性状与SSR标记的相关性分析*
Correlation analysis of SSR markers with growth-related traits of blood clam (Tegillarca granosa)
投稿时间:2019-04-17  修订日期:2019-06-26
DOI:
中文关键词: 泥蚶  SSR标记  生长性状  相关分析
英文关键词: Tegillarca granosa  EST-SSR marker  growth trait  correlation analysis
基金项目:国家重点研发专项(2018YFD0901405); 国家水产种质资源共享服务平台(2018DKA30470); 浙江省科技计划项目(2017F30042).
作者单位E-mail
邵艳卿① 浙江省海洋水产养殖研究所 amshao@126.com 
方 军 浙江省海洋水产养殖研究所  
田 野 浙江省海洋水产养殖研究所  
滕爽爽 浙江省海洋水产养殖研究所  
张雷雷 浙江省海洋水产养殖研究所  
柴雪良① 浙江省海洋水产养殖研究所 cxl-5888@163.com 
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中文摘要:
      泥蚶生长性状属于数量性状,为了筛选与泥蚶生长性状位点连锁的分子标记,本研究以150颗选系G4泥蚶为研究材料,利用12个SSR位点进行生长性状关联分析。对生长性状的相关分析表明,各数量性状之间的相关系数均达到极显著水平(P < 0.01),其中壳高与活体重的相关系数最大(0.930),壳长与活体重次之(0.927);但通径分析显示,对活体重直接影响最大的是壳宽(0.431),壳长次之(0.332),决定系数与以上通径分析结果的变化趋势一致。应用SSR位点对选系G4泥蚶进行遗传多样性分析,共检测到61个等位基因,等位基因数(Na)为3~8个,平均等位基因和有效等位基因(Ne)分别为5.083和3.038;平均观测杂合度(Ho)、期望杂合度(He) 和多态信息含量(PIC)分别为0.528、0.646和0.594,表明该群体遗传多样性处于较高水平。SSR位点与生长性状相关性分析,结果获得3个标记与生长性状具有显著相关性(P < 0.05),其中标记3012-2与壳长、壳宽、壳顶宽显著相关(P < 0.05),3564与壳高、放射肋宽显著相关(P < 0.05),2692与壳长显著相关(P < 0.05),据此初步确定为与生长性状相关的候选标记。本研究旨为泥蚶的遗传改良和选择育种提供基础资料。
英文摘要:
      Growth traits of blood clam (Tegillarca granosa) is quantitative traits. In order to acquire some reliable molecular genetic markers for growth-related traits, the correlation analysis of 12 SSR markers and growth-related traits in T. granosa were analyzed using 150 samples with the fourth generation selected line of blood clam. And the correlation and path analysis method were used to analyze the effect of phenotypic character on qualitative traits. Then results showed that the correlations among the qualitative characters reached extremely significant levels (P < 0.01). The shell height showed the greatest correlation coefficients to the live weight (0.930). However, the result of the path coefficient analysis showed that the highest path coefficients were the shell width (0.432). The results of the determinant coefficients were consistent with the results of the path analysis. In addition, a total of 61 alleles for the 12 loci were detected (3~8 alleles for each locus), and the average number of alleles and effective number of alleles were 5.083 and 3.038, respectively. The average observed heterozygosity (Ho), expected heterozygosity (He) and polymorphism information content (PIC) was 0.528, 0.646, and 0.594, respectively, both of which indicated that the population genetic diversity was high. Correlation analysis between genotypes of SSR and growth traits indicated that 3012-2 had a significant impact on shell length, shell width and shell umbo width ( P<0.05). Locus 3564 showed a significant influence on shell height and radial rib width ( P<0.05). Locus 2692 showed a significant correlation with shell length. Our results could provide an theoretical basis for the study of genetic improvement and selective breeding of T. granosa.
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