文章摘要
大菱鲆脂质代谢相关基因PPARs的组织表达及其对高温胁迫的响应
Tissue expression of lipid metabolism related genes PPARs and their responses to heat stress in turbot (Scophthalmus maximus)
投稿时间:2019-04-18  修订日期:2019-06-05
DOI:
中文关键词: 大菱鲆(Scophthalmus maximus)  脂质代谢  过氧化物酶体增殖物激活受体(PPARs)  基因表达  高温胁迫
英文关键词: turbot (Scophthalmus maximus)  heat stress  lipid metabolism  peroxisome proliferator activated receptors (PPARs)  gene expression
基金项目:现代农业产业技术体系建设专项(CARS-47-01);青岛海洋科学与技术试点国家实验室“鳌山人才”培养计划项目(2017ASTCP-OS04);国家自然科学基金项目41706168;山东省农业良种工程项目(2016LZGC031);国家重点研发计划2018YFD0900102.
作者单位E-mail
赵亭亭 中国水产科学研究院黄海水产研究所 Zhaotingting0128@163.com 
杨双双 青岛海洋科学与技术试点国家实验室
中国水产科学研究院黄海水产研究所 
 
马爱军 青岛海洋科学与技术试点国家实验室
中国水产科学研究院黄海水产研究所 
maaj@ysfri.ac.cn 
黄智慧 青岛海洋科学与技术试点国家实验室
中国水产科学研究院黄海水产研究所 
 
孙志宾 青岛海洋科学与技术试点国家实验室
中国水产科学研究院黄海水产研究所 
 
王新安 青岛海洋科学与技术试点国家实验室
中国水产科学研究院黄海水产研究所 
 
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中文摘要:
      过氧化物酶体增殖物激活受体(PPARs)是一类核激素超家族受体,存在PPARα、PPARβ和PPARγ 3种亚型,是控制脂质代谢的主要核受体之一,在调节脂质代谢相关基因的转录中起到重要作用。实验采用荧光定量PCR(qPCR)技术检测PPARs基因3种亚型在大菱鲆不同组织中的表达情况以及高温胁迫下大菱鲆肾脏中PPARs的表达情况。qPCR结果显示,大菱鲆PPARs 3种亚型的组织分布存在明显差异。PPARα1和PPARα2在心脏中表达量显著高于其他组织;PPARβ在大菱鲆的各个组织中普遍表达;PPARγ在大菱鲆的鳃中出现了显著性的高表达。大菱鲆肾脏PPARs的mRNA水平随着温度升高呈现出不同的响应模式。PPARα随温度升高表达水平先显著降低,后有所升高;PPARβ的表达量在14、20、23和25℃时差异不显著,当温度升高至大菱鲆的致死温度28℃时,表达量出现了显著性的升高;PPARγ在14℃时表达水平很低,但随着温度的升高不断增加。研究表明,大菱鲆中存在PPARα、PPARβ和PPARγ 3种亚型,而且三者可能以组织特异性的方式参与脂类代谢的调节,首次指出PPARs 3种亚型在温度胁迫中的表达变化,对PPARs的研究将推动鱼类脂代谢研究的进一步深入,揭示鱼类PPARs在脂质代谢调控以及响应逆境胁迫中的重要作用。
英文摘要:
      Scophthalmus maximus is a commercially valuable flatfish and one of most promising aquaculture species in Europe. Since Scophthalmus maximus was introduced into China in 1990s, it has been a major maricultured fish in northern coastal areas of China. As a kind of cold-water fish, Scophthalmus maximus has strict requirements on breeding temperature, which greatly limits the breeding range of this important marine fish. Therefore, it has great research significance to explore the mechanism of heat-resistance in turbot. In this paper, tissue distribution of PPARs and the expression of PPARs in kidney under different temperature were analyzed by qPCR. The results showed that there are significant differences in the tissue distribution of the three subtypes of Scophthalmus maximus PPARs. PPARα1 and PPARα2 were markedly expressed in the heart, PPARβ was widely distributed in various tissues of Scophthalmus maximus, while PPARγ was significantly expressed in the gill of Scophthalmus maximus. What's more, the transcript abundance of PPARs in Scophthalmus maximus kidney showed different response patterns with the elevated temperature. At 20°C, the expression level of PPARα diminished dramatically and then increased with the elevated temperature. The expression of PPARβ was not significantly different at 14, 20, 23 and 25°C, however, its transcript abundance increased significantly when the temperature was increased to the lethal temperature of Scophthalmus maximus at 28°C. The expression level of PPARγ showed a tendency to increase with increasing temperature. In summary, our results revealed that there are three subtypes PPARα, PPARβ and PPARγ in Scophthalmus maximus, which may be involved in the regulation of lipid metabolism in a tissue-specific manner, and more importantly, the expression of three subtypes of PPARs in thermal stress was pointed out for the first time. Collectively, these studies will shed light on the regulation mechanism of PPARs on lipid metabolism in the kidney of Scophthalmus maximus during heat stress, which will promote the further study of fish lipid metabolism.
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