文章摘要
池塘种植鱼腥草对罗非鱼感染无乳链球菌后肝脏抗氧化性能、组织病理和HSP70表达的影响
Effect of pond-planted houttuynia cordata on hepatic antioxidative abilities, histopathology and HSP70 expression in Oreochromis spp. infected by streptococcus agalactiae
投稿时间:2019-05-07  修订日期:2019-07-09
DOI:
中文关键词: 鱼腥草;罗非鱼  无乳链球菌  组织病理  热休克蛋白70
英文关键词: houttuynia cordata, tilapia(Oreochromis spp.), Streptococcus agalactiae, histopathology, HSP70
基金项目:现代农业产业技术体系建设专项 (CARS-46);中央级公益性科研院所基本科研业务费专项(2017JBFZ03);水产原良种保种选育及亲本更新(2130135)
作者单位E-mail
祝璟琳 中国水产科学研究院淡水渔业研究中心 zhujl@ffrc.cn 
季桓涛 南京农业大学无锡渔业学院  
肖炜 中国水产科学研究院淡水渔业研究中心  
李大宇 中国水产科学研究院淡水渔业研究中心  
邹芝英 中国水产科学研究院淡水渔业研究中心  
杨 弘 中国水产科学研究院淡水渔业研究中心 yanghong@ffrc.cn 
李庆勇 广东省惠州市渔业研究推广中心  
朱德兴 广东省惠州市渔业研究推广中心  
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中文摘要:
      为探讨池塘种植鱼腥草(Houttuynia cordata)对罗非鱼(OreochromisSSspp)感染无乳链球菌后降低了死亡率的原因,在养殖池塘中分别种植0%(对照组)、5%、10%、15%比例池塘面积的鱼腥草,养殖90 d后进行无乳链球菌人工感染,分别在感染后0、24、48和72 h采集肝脏,进行抗氧化性能、组织病理和HSP70表达研究。结果显示:感染后48h和72h对照组肝脏ALT和AST活力最高,种植鱼腥草各组ALT活力在感染前后都没有显著性变化,感染后72h 10%组AST活力已恢复到感染前水平。抗氧化指标显示鱼腥草种植组肝脏GSH-PX和CAT活力在感染后都显著性升高;10%组的罗非鱼肝脏NOS活力在感染后48h显著升高;15%组罗非鱼肝脏SOD活力在感染后48h显著性升高;感染前肝脏MDA含量随鱼腥草种植面积的增加而显著性降低;感染后48h对照组罗非鱼肝脏T-AOC活力显著性降低,此时各种植鱼腥草组没有显著性变化。组织病理学观察显示对照组罗非鱼感染后48h 肝窦明显淤血,肝索排列紊乱,肝细胞脂肪变性,而各种植鱼腥草组罗非鱼在感染后仅表现为肝细胞明显嗜酸,肝窦轻度扩张,未见明确炎症反应及组织坏死。定量PCR结果显示每组罗非鱼肝脏HSP70 mRNA表达量在感染后都显著性升高,感染后各时间点对照组的HSP70表达量都显著高于鱼腥草种植组,感染后72h 10%组显著性高于5%组和15%组。研究表明种植鱼腥草能显著改善链球菌感染所造成的肝AST、ALT上升,使罗非鱼肝脏中抗氧化酶的活性升高,抑制脂质过氧化MDA的产生,减缓无乳链球菌感染引起的总抗氧化能力下降,显著减轻链球菌感染引起病理损伤;通过HSP70表达降低炎症反应,保持炎症平衡,初步阐明了种植鱼腥草减少链球菌感染引起的罗非鱼死亡的机制。
英文摘要:
      The present study was conducted to investigate the effects of pond-planted houttuynia cordata levels on hepatic antioxidative status, histopathology and HSP70 expression in Oreochromis spp. infected by streptococcus agalactiae. Four groups of different proportions of Houttuynia cordatah were set. They were 0%(as control), 5%, 10% and 15% area of ponds. After 90 days cultivation, All fish were challenged by S. agalactiae. Liver samples were collected at 0, 24, 48 and 72 hour post injection(hpi). The results showed that hepatic alanine transaminase(ALT) and aspartate aminotransferase(AST) activity reached the peak in the control group at 48 and 72 hpi respectively, and there was no significant change in ALT activity before and after infection in houttuynia cordata-planted groups. The AST activity recovered to the pre-infection level at 72 hpi in 10% group. Antioxidant indexes showed that the activity of glutathione peroxidase (GSH-PX) and catalase (CAT) in houttuynia cordata-planted groups increased significantly after infection. Hepatic NOS activity increased significantly at 48 hpi in 10% group, while hepatic superoxide dismutase (SOD) activity raised significantly at 48 hpi in 15% group. Hepatic malonyldialdehyde (MDA) decreased significantly with the increase of planting area of houttuynia cordata before infection. The hepatic total antioxidative capacity(T-AOC) in the control group reduced significantly at 48 hpi, while there was no significant change in houttuynia cordata-planted groups. Histopathology showed that hepatic sinusoid congested obviously, hepatic cord arranged irregularly, hepatocyte macrovesicular steatosis at 48 hpi in the control group, while the tilapia in houttuynia cordata-planted groups showed only obvious eosinophilic and the hepatic sinus is slightly dilated. There were no obvious inflammatory reaction or tissue necrosis. Quantitative PCR results showed that the expression level of HSP70 mRNA in the liver of each group increased significantly after infection. The expression level of HSP70 in the control group at each time point was significantly higher than that in the houttuynia-planted groups after infection. And HSP70 expression level in the 10% group was significantly higher than that in the 5% and 15% groups at 72 hpi. The results of this study suggest that planting houttuynia cordate can significantly alleviate hepatic AST, ALT rising, increased activity of antioxidant enzymes, reduce hepatic MDA level, and maintain activity of T-AOC. And it alleviated the pathological damage caused by streptococcus infection. The expression of HSP70 can reduce the inflammatory response and maintain the balance of inflammation. The paper preliminarily elucidated the mechanism of planting houttuynia cordata to reduce the death of tilapia challenged by S. agalactiae.
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