文章摘要
养殖银鲳虹彩病毒感染的组织病理学分析及分子检测
Histopathological analysis and molecular detection of iridovirus in cultured silver pomfret
投稿时间:2019-05-11  修订日期:2019-07-28
DOI:
中文关键词: 养殖银鲳  肿大细胞病毒  虹彩病毒  分子诊断
英文关键词: Pampus argentens  Megalocytivirus  Iridovirus  Molecular diagnosis
基金项目:宁波市农业重大专项(2015C110003);浙江省重大科技专项项目(2018C02G2070580)
作者单位E-mail
许跃 宁波大学海洋学院生物技术教育部重点实验室 1016130431@qq.com 
钱冬 宁波大学海洋学院生物技术教育部重点实验室  
周素明 宁波大学海洋学院生物技术教育部重点实验室 zhousuming@nbu.edu.cn 
王亚军 宁波大学海洋学院生物技术教育部重点实验室  
尹飞 宁波大学海洋学院生物技术教育部重点实验室  
金珊 宁波大学海洋学院生物技术教育部重点实验室  
詹萍萍 宁波大学海洋学院生物技术教育部重点实验室  
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中文摘要:
      2018年7月宁波象山某养殖场内银鲳(Pampus argentens)出现集中死亡现象, 累积死亡率高达80%以上。患病银鲳临床表现为厌食、身体失衡、脾脏肿大、肝脏颜色异常的病征。组织病理学观察发现患病鱼肝脏、脾脏、肾脏均出现直径在10~15μm大小的嗜碱性肿大细胞。进一步的透射电镜观察则在脾肾等组织细胞内发现了病毒包涵体结构以及大量的的病毒样粒子,直径为140~160nm。通过虹彩病毒特异性PCR检测发现患病组织样品为虹彩病毒阳性。此外,通过病毒主要衣壳蛋白(MCP)基因序列分析,发现银鲳源病毒与大黄鱼虹彩病毒(Genbank 登入号:AY779031.1)MCP同源性最高为99.76%,认为它们属于虹彩病毒科 (Iridovirus)细胞肿大病毒属(Megalocytivirus) 真鲷虹彩病毒(red sea bream iridovirus, RSIV)类群。本文首次报道了全人工养殖环境下银鲳感染虹彩病毒的病例,该研究将为养殖银鲳虹彩病毒病的诊断和防治提供了重要的参考依据。
英文摘要:
      In July 2018, an outbreak of unknown disease occurred in the silver pamfret (Pampus argentens) cultured in a farm in Xiangshan, Ningbo, with a cumulative mortality rate of over 80%. The clinical manifestations of sick silver carp are anorexia, body imbalance, swelling of the spleen, and abnormal color of the liver. Histopathological analysis showed the presence of basophilic enlarged cells with a diameter of 10~15μm in diseased fish spleen, liver and kidney. Further transmission electron microscopic observation revealed the presence of inclusion bodies and a large number of virus-like particles (a diameter of 140-160 nm) in cells from spleen and kidney tissues. Moreover, results of the iridovirus-specific PCR test showed that all these tissue sample collected from diseased fish were positive of iridovirus. To further detect the presence of iridovirus, the major capsid protein (MCP) gene of virus in silver pomfret was amplified, cloned and sequenced. Sequence similarity analysis showed that silver pomfret-derived virus (SPDV) shared the highest homology with the large yellow croaker iridovirus (LYCIV) (GenBank accession number: AY779031.1 ) MCP, with a sequence similarity of 99.76%. In a phylogenetic tree, SPDV and LYCIV are closely grouped with red sea bream iridovirus (RSIV) strains. Thus, we conclude that the virus infected silver pomfret belongs to the Iridovirus family, Megalocytivirus genus, RSIV group. In summary, this paper documented the first case of Megalocytivirus infection in cultured silver pomfret. This study will provide an important reference for the diagnosis and prevention of silver carp iridescent virus disease.
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