文章摘要
饲料中维生素A对青鱼幼鱼生长、血清生化指标和肝脏糖脂代谢酶活性及基因表达的影响
Effect of vitamin A on growth, serum biochemical index, digestive enzyme activities and glucose and lipid metabolism in juvenile black carp Mylopharyngodon piceus
投稿时间:2019-05-13  修订日期:2019-05-28
DOI:
中文关键词: 维生素A  青鱼  生化指标  糖脂代谢  基因表达
英文关键词: vitamin A  Mylopharyngodon piceus  biochemical index  glucose and lipid metabolism  gene expression
基金项目:国家大宗淡水鱼产业技术体系岗位科学家任务(CARS-45-11),国家自然科学基金面上项目(31672669)
作者单位E-mail
陈书健 宁波大学海洋学院 shujianchen94@163.com 
吴成龙 湖州师范学院生命科学学院  
叶金云 湖州师范学院生命科学学院 ziff2006@163.com 
李荣华 宁波大学海洋学院  
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中文摘要:
      摘要:为研究饲料中维生素A(VA)对青鱼幼鱼生长、血清生化指标、和肝脏糖脂代谢相关酶活性及基因表达的影响。以初始体重为6.10±0.10 g的青鱼幼鱼为研究对象,以无维酪蛋白和明胶为蛋白源,菜籽油为脂肪源,糊精为糖源,分别配置含三个VA醋酸酯水平(添加量为0,2200和20000 IU/kg,实测值为178.2 IU/kg,2058.9 IU/kg和18436.2 IU/kg)的试验饲料,养殖周期为8周。结果显示:VA缺乏显著降低青鱼幼鱼增重率(WG)和特定生长率(SGR)(P<0.05);VA缺乏会显著降低血清血糖(Glu)、甘油三酯(TG)和低密度脂蛋白(LDL)浓度,增加总胆固醇(TCH)浓度(P<0.05)。饲料中添加2058.9 IU/kgVA能显著提高肝脏己糖激酶(HK)、磷酸果糖激酶(PFK)、丙酮酸激酶(PK)活性,促进葡萄糖转运蛋白-2(GLUT-2)、HK、葡萄糖激酶(GK)、PFK和葡萄糖-6-磷酸酶(G6Pase)基因表达(P<0.05)。饲料中VA含量为2058.9 IU/kg时,对肝脏FATP-1基因表达无显著影响(P>0.05),显著影响肉碱棕榈酰转移酶-1(CPT-1)和肉碱棕榈酰转移酶-2(CPT-2)基因表达(P<0.05)。当饲料中VA缺乏时,CPT-1和CPT-2基因表达受到显著性抑制(P<0.05)。当饲料中添加过量VA时,乙酰辅酶A羧化酶-2(ACC-2)和脂蛋白脂酶(LPL)基因表达受到抑制(P<0.05)。同时,VA过量组中肝脏过氧化物酶体增殖激活受体-γ(PPAR-γ)基因表达显著下降(P<0.05)。试验结果表明:在饲料中添加2058.9 IU/kg VA可以促进青鱼幼鱼生长,提高肝脏对葡萄糖的转运能力,促进糖酵解和糖异生代谢平衡,同时促进脂肪酸合成和转运。
英文摘要:
      Abstract: An 8-week feeding trail was carried out to investigate the effect of dietary vitamin A (178.2, 2058.9, 18436.2 IU/kg) on growth performance, serum biochemical index and hepatic glucose and lipid metabolism key enzyme activities and gene expression levels of juvenile black carp (Mylopharyngodon piceus). Three isonitrogenous and isoenergetic purified diets with different levels of vitamin A diets were formulated. Total 360 juvenile black carp with initial weight 6.10 ± 0.10 g were randomly divided into 3 groups with 3 replicates per group and 40 black carp per replicate. The results showed as follows: There was no significant difference on hepatosomatic index (HSI) and condition factor (CF) in all groups (P>0.05). However, weightgain rate (WG) and specific growth rate (SGR) were reduced for fish fed diet with 178.2 IU/kg VA (P<0.05). When VA is deficient, concentrations of serum glucose, triglycerides and Low-density lipoprotein (LDL) were decreased, buttotal cholesterol (TCH) concentration was increased (P<0.05). The activities of hexokinase (HK), phosphofructokinase (PFK) and pyruvate (PK) increased for fish fed diet with 2058.9 IU/kg VA. Increased gene expression of liver Glucose transporter-2 (GLUT-2), HK, GK, PFK and G6Pase were also observed in fish fed diet with 2058.9 IU/kg VA. However, there were no significant difference on the gene expression of fatty acid transporter protein-1 (FATP-1) for fish fed diet with 2058.9 IU/kg VA (P>0.05). But the gene expression of Carnitine O-palmitoyltransferase-1 (CPT-1) and Carnitine O-palmitoyltransferase-2 (CPT-2) were significantly influenced (P<0.05). The gene expression of CPT-1 and CPT-2 were inhibited for fish fed VA deficient diet (P<0.05). When fish fed diet with 18436.2 IU/kg VA, the expression of acetyl-CoA carboxylase-2 (ACC-2) and lipoprotein lipase (LPL) genes were inhibited. Besides, the gene expression of Peroxisome proliferator-activated receptor-γ (PPARγ) decreased when the VA content was 18436.2 IU/kg in feed. Overall, fish fed diet with 2058.9 IU/kg VA could improve growth performance and ability of liver cells to transport glucose of juvenile black carps. Diet with 2058.9 IU/kg VA could keep a balance between glycolysis and gluconeogenesis, it also promotes fatty acids synthesis and transport.
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