文章摘要
斜带石斑鱼蛋白质沉积和免疫相关基因表达与饲料精氨酸水平的响应
Effects of dietary arginine levels on growth, immunity and gene expression in orange-spotted grouper (Epinephelus coioides)
投稿时间:2019-05-15  修订日期:2019-06-24
DOI:
中文关键词: 精氨酸  斜带石斑鱼  蛋白沉积  免疫基因表达
英文关键词: arginine  Epinephelus coioides  protein deposition  immune gene expression
基金项目:广东省自然科学基金项目(2017A030313155),农业部现代农业产业技术体系专项资金(CARS-47)资助
作者单位E-mail
崔晓 广东海洋大学水产动物营养与饲料实验室 976605133@qq.com 
韩凤禄 广东海洋大学水产动物营养与饲料实验室  
迟淑艳 广东海洋大学水产动物营养与饲料实验室 chishuyan77@163.com 
谭北平 广东海洋大学水产动物营养与饲料实验室  
董晓慧 广东海洋大学水产动物营养与饲料实验室  
杨奇慧 广东海洋大学水产动物营养与饲料实验室  
刘泓宇 广东海洋大学水产动物营养与饲料实验室  
章双 广东海洋大学水产动物营养与饲料实验室  
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中文摘要:
      本试验旨在研究饲料中不同水平精氨酸对斜带石斑鱼(Epinephelus coioides)(初重80.11 ± 0.03)g生长性能和免疫的影响。配制7种等氮等脂的试验饲料,精氨酸浓度分别为2.13%、2.42%、2.71%、2.95%、3.20%、3.48%和3.74%。随机挑选健康的石斑鱼分成7组,每组设3个重复,每个重复25尾鱼,进行为期10周的养殖试验。结果显示:2.71%组鱼体增重率和特定生长率显著高于2.13%和2.42%组(P<0.05),饲料系数显著低于2.13%组(P<0.05)。以特定生长率为判据,经折线模型拟合可得出,斜带石斑鱼对饲料中精氨酸最适需求量为饲料的2.72% (饲料蛋白的5.40%)。3.20%血清胰岛素(INS)与3.48%组差异不显著(P>0.05),显著高于其他组(P<0.05)。3.48%组鱼体肌肉TOR mRNA水平显著高于2.13%、2.42%和2.71%组(P<0.05)。2.42%组后肠b0,+ AT基因表达量最高,显著高于其他各组(P<0.05)。2.95%和3.28%组肾脏b0,+ AT基因表达量差异不显著(P>0.05),显著高于其他组(P<0.05)。综上,精氨酸可以刺激IGF-I的分泌,促进蛋白质的合成,提高机体免疫力,促进鱼体生长。
英文摘要:
      An 10 weeks feeding trial was to investigate the effects of dietary arginine levels on the growth performance, serum biochemical indexes, immune genes, and amino acid transporter b 0,+ AT . Seven isonitrogenous and isolipid commercial diets were formulated with arginine contents of 2.13%, 2.42%, 2.71%, 2.95%, 3.20%, 3.48% and 3.74% (dry matter). Randomly selected healthy groupers(initial weight:S80.11±0.03g)were divided into 7 groups, with 3 replicates in each group and 25 fish per replicate. It was found that the weight gain rate(WGR)and specific growth rate(SGR)of 2.71% group were the highest and the feed conversion ratio(FCR)was the lowest. Broken-line model analysis of specific growth rate against dietary arginine levels indicated that the optimum requirement of arginine for maximum growth of subadult grouper was 2.72%, corresponding to 5.40% of dietary protein on a dry weight basis. There was no significant difference between 3.20% serum insulin (INS) and 3.48% group (P > 0.05), which was significantly higher than other groups (P < 0.05). The level of TOR gene in muscle of 3.48% group was significantly higher than that of 2.13%, 2.42% and 2.71% group (P < 0.05). The expression of b0, +AT gene in the hindgut of 2.42% group was the highest, which was significantly higher than that of other groups (P < 0.05). There was no significant difference in the expression of b0, + gene between 2.95% and 3.28% groups (P > 0.05), which was significantly higher than that of other groups (P < 0.05). In conclusion, arginine can stimulate the secretion of IGF-I, promote protein synthesis, improve immunity and promote fish growth.
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