文章摘要
急性氨氮胁迫对黄颡鱼组织抗氧化酶活性及HSP70和HSP90基因mRNA表达水平的影响
Effect of acute ammonia stress on antioxidant enzyme activity and mRNA expression levels of HSP70 and HSP90 genes in tissues of yellow catfish Pelteobagrus fulvidraco
投稿时间:2019-05-30  修订日期:2019-10-23
DOI:
中文关键词: 黄颡鱼  氨氮  抗氧化酶  HSP70  HSP90
英文关键词: yellow catfish  ammonia nitrogen  antioxidant enzyme  HSP70  HSP90
基金项目:国家自然科学基金
作者单位E-mail
孙丽颖 宁波大学海洋学院 1304326280@qq.com 
张木子 宁波大学海洋学院  
黎明 宁波大学海洋学院 liming1@nbu.edu.cn 
袁莉霞 宁波大学海洋学院  
王日昕 宁波大学海洋学院  
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中文摘要:
      为了探讨急性氨氮胁迫对黄颡鱼组织中抗氧化酶活性及HSP70和HSP90基因 mRNA表达水平的影响,本实验随机挑选了360尾黄颡鱼(初体重17.25 ± 0.05)g,分别暴露于含有0(对照),5.70,28.50和57.00 mg/L总氨氮浓度的水体中,开展为期96 h的急性胁迫实验。实验开始后,分别于0,12,24,48和96 h取样。结果显示,氨氮胁迫发生后,低、中浓度组实验鱼肝脏中超氧化物歧化酶(SOD)活性呈先升高后降低趋势,而高浓度组则持续降低;低、中、高浓度组实验鱼肝脏中丙二醛(MDA)含量在胁迫开始后显著升高;3 h时,高浓度组实验鱼肝脏中SOD活性达到最低,而MDA含量最高;24 h后,高浓度组实验鱼肝脏中过氧化氢酶(CAT)活性显著升高;低、中、高浓度组实验鱼肝脏中HSP70基因的mRNA表达量呈先降低后升高趋势,而鳃中HSP70基因表达量持续升高,但脑中HSP70基因在0 h后显著降低;氨氮胁迫3 h时,低、中、高浓度组实验鱼肝脏和脑中HSP70基因表达量显著低于对照组,而在鳃中正好相反;相比HSP70基因,高浓度组实验鱼肝脏和鳃中HSP90基因的mRNA表达量在24 h时达到最高。结果显示,不同浓度的氨氮胁迫会对黄颡鱼抗氧化酶活性造成不同程度的抑制,原因与丙二醛的积累量有关;相比HSP90基因,黄颡鱼HSP70基因的表达量在氨氮胁迫发生后迅速上调,而这种生理调控机制提示,HSP70在应对急性氨氮胁迫时,发挥着更重要的作用。
英文摘要:
      A study was carried out to test the effect of acute ammonia stress on antioxidant enzyme activity and mRNA expression levels of HSP70 and HSP90 genes in tissues of yellow catfish, 360 yellow catfishes (17.25 ± 0.05) g were randomly exposed to ammonia: 0 (control), 5.70, 28.50 and 57.00 mg/L total ammonia concentrations for 96 h, respectively. Experimental fish were sampled on 0, 12, 24, 48 and 96 h. The results showed that the superoxide dismutase (SOD) activity in liver of fish in low and medium concentration groups increased from 0 to 6 h, and then gradually decreased, but high concentration group continued to decrease. Malondialdehyde (MDA) content in liver of fish in low, medium and high concentration group was significantly increased throughout the 96-h period; At hour 3, SOD activity in liver of fish in high concentration group was the lowest, while MDA content was the highest. Liver catalase activity increased significantly at hour 24. In low, medium and high concentration groups, the mRNA expression of HSP70 in liver of fish decreased from 0 to 6 h, and then gradually increased, and in gill of fish continued to increase, but in brain of fish continued to decreased. The mRNA expression of HSP70 in liver and brain of fish was significantly lower than that of control group at hour 3, but in gills is opposite. Compared with HSP70, the mRNA expression of HSP90 in liver and gill of fish in high concentration group reached the highest value at hour 24. The results showed that the antioxidant enzymes activity was inhibited by different concentrations of ammonia, the reason is related to the accumulation of MDA. Compared with HSP90, the mRNA expression of HSP70 in yellow catfish was rapidly up-regulated after the occurrence of ammonia stress, and this physiological regulation mechanism suggests that HSP70 plays a more important role in the response to acute ammonia stress.
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