| In October 2018, a crucian carp (Carassius auratus) farm located in Zigong, Sichuan Province, developed a communicable disease with a mortality rate of 80%. 【Purpose】In order to explore the cause of this disease, 【Method】sick fish were subjected to necropsy, pathological observation, molecular biological examination and artificial infection test. 【Results】 The clinical manifestations of diseased fish include leave the shoal of fish to swim alone; stay on the water surface; bodies blacken and body surface have haemorrhages. The necropsy showed haemorrhage, swelling and necrosis of organs such as gills, liver and kidney; swimming bladder have a lot of blutene chloaide. Histopathological changes show that the respiratory epithelial cells of gill were swollen, necrosis and sloughting. The kidneys show focal necrosis. The spleen tissue was extensively degenerated and necrotic, and the hematopoietic system collapses. Vacuolar degeneration and necrosis occur in the liver. Transmission electron microscopy showed that there were four different sizes of virus particles in the spleen and kidney, which were virus DNA core, empty viral capsid, solid capsid and enveloped mature virions. It can be seen that immature virions are present in the nucleus, and mature virions are present in the cytoplasm. Cyprinid herpesvirus Ⅱ (CyHV-2) completes nucleic acid replication and nucleocapsid assembly in the nucleus, and the envelope protein is obtained after transmembrane membrane. After filtering the diseased fish tissue homogenate, it was inoculated into EPC and FHM cell lines, and there was no cytopathic effect after 3 generations of blind transmission. The helicase gene fragment of CyHV-2 was amplified by PCR, and a positive band appeared. The artificial infection test was carried out by intraperitoneal injection of tissue homogenate. The test group showed the same clinical symptoms as the natural infection fish and the control group was normal. The cumulative mortality rate of the test group was 80%. Phylogenetic analysis of helicase gene by the neighbor-joining method revealed that the homology with CyHV-2 (YZ-01) was 99%. 【Conclusion】The results demonstrated that CyHV-2 was contributed to this outbreak disease. This study reported an outbreak disease caused by CyHV-2 in cultured crucian carp in Sichuan and systematic pathological was studied, which provided an important basis for study the pathogenesis and epidemiology of CyHV-2.