文章摘要
饲料n-3 HUFA水平对珍珠龙胆石斑鱼生长、免疫力及相关基因表达和抗病力的影响
WEffects of dietary n-3 highly unsaturated fatty acids on growth, immunity and gene expression and disease resistance of hybrid grouper (♀ Epinephelus fuscoguttatus × ♂ Epinephelus lanceolatu)
投稿时间:2019-08-31  修订日期:2019-09-30
DOI:
中文关键词: n-3高不饱和脂肪酸,非特异性免疫,炎症相关基因,珍珠龙胆石斑鱼
英文关键词: n-3 HUFA  non-specific immunity  inflammatory related gene  ♀Epinephelus fuscoguttatus×♂Epinephelus lanceolatu
基金项目:国家自然科学基金,
作者单位E-mail
安文强 广东海洋大学水产学院水产动物营养与饲料实验室 1005451975@qq.com 
董晓慧 广东海洋大学水产学院  
谭北平 广东海洋大学水产学院  
杨奇慧 广东海洋大学水产学院  
迟淑艳 广东海洋大学水产学院  
章双 广东海洋大学水产学院  
刘泓宇 广东海洋大学水产学院  
杨原志 广东海洋大学水产学院  
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中文摘要:
      本研究旨在研究饲料不同n-3 高不饱和脂肪酸(HUFA)水平对珍珠龙胆石斑鱼幼鱼生长性能、非特异性免疫、免疫相关基因表达及对抵抗哈维氏弧菌(Vibrio harveyi)能力的影响。配制n-3 HUFA水平分别为0.65%(对照组),1.00%,1.35%,1.70%,2.05%和2.40% 的6种等氮等脂的实验饲料,投喂初始体重为12.06 ± 0.01g的珍珠龙胆石斑鱼幼鱼8周。结果表明:1)饲料n-3HUFA水平对饲料系数(FCR)、存活率(SR)、肝体比(HSI)和肥满度(CF)均无显著性影响(P>0.05);1.35%组增重率(WG)和特定生长率(SGR)显著高于2.40%组(P<0.05)。2)1.00%组的体粗脂肪显著低于1.70%和2.40%组(P<0.05)。肝脏和肌肉脂肪酸的组成反映饲料脂肪酸的组成。3)攻毒前,1.35%和1.70%组血清超氧化物歧化酶(SOD)、过氧化氢酶(CAT)的活性和补体C3的浓度显著高于对照组(P<0.05)。攻毒后,血清CAT、谷胱甘肽过氧化物酶(GSH-PX)、溶菌酶(LZM)活性和C3含量急剧上升,SOD活性显著下降(P<0.05)。攻毒前,2.40%组肠道TLR22和MyD88 mRNA的表达量显著增加,2.05%组TNF-α和IL-1β的表达量显著高于1.00%和1.35%组(P<0.05);此外,1.70%组肾脏TLR22和IL-1β的表达量显著低于对照组(P<0.05)。攻毒后,1.35%组肠道MyD88 mRNA的表达量显著高于1.00%和1.70%-2.40%组(P<0.05),1.70%组TNF-α和IL-1β的表达量有最小值;1.70%组肾脏IL-10的表达量显著高于其它各组,而IL-1β的表达量呈相反趋势,显著低于2.40%组(P<0.05)。本研究结果表明,适宜的饲料n-3 HUFA(1.47%-1.70%)可以提高珍珠龙胆石斑鱼的生长性能、非特异性免疫力及抑制肠道炎症反应。
英文摘要:
      This study was conducted to investigate the effects of dietary n-3 highly unsaturated fatty acids (n-3 HUFA) on growth performance, non-specific immunity, expression of some immune-related genes and resistance to Vibrio harveyi in juvenile hybrid grouper (♀ Epinephelus fuscoguttatus × ♂ Epinephelus lanceolatu). Six isoproteic and isolipidic experimental diets were formulated with graded levels of n-3 HUFA (0.65, 1.00, 1.35, 1.70, 2.05 and 2.40% of dry matter, respectively), and the 0.65% group was used as control group. Each diet was randomly allocated to triplicate groups of fish in 1000 L fiberglass tank, and each tank was stocked with 40 fish (initial weight 12.06 ± 0.01 g) for 8 weeks. Results showed that feed conversion ratio (FCR), survival rate (SR), hepatosomatic index (HSI) and condition factor (CF) were all not significantly affected by dietary n-3 HUFA levels (P > 0.05). Weight gain (WG) and specific growth rate (SGR) in 1.35% group were significantly higher than those in 2.40% group (P < 0.05). Crude lipid of body in 1.00% group was significantly lower than that in 1.70% and 2.40% groups (P < 0.05). Liver and muscle fatty acid profiles reflected that of diets. Before challenge, the activity of serum superoxide dismutase (SOD), catalase (CAT) and content of complement 3 (C3) in 1.35% and 1.70% groups significantly higher than those of control group (P < 0.05). After challenge, serum CAT, glutathione peroxidase (GSH-PX), lysozyme (LZM) and C3 all increased sharply, while SOD showed the opposite trend. Before challenge, the expression levels of intestine toll-like receptor 22 (TLR22) and myeloid differentiation factor 88 (MyD88) mRNA in 2.40% group were significantly increased, and the expression levels of tumour necrosis factor α (TNF-α) and interleukin 1β (IL-1β) mRNA in 2.05% group were significantly higher than those in 1.00% and 1.35% groups (P < 0.05). In addition, the TLR22 and IL-1β mRNA levels in kidney of 1.70% group were significantly lower than those in control group (P < 0.05). After challenge, the expression level of MyD88 mRNA in intestine of 1.35% group was significantly higher than that in 1.00% group and from 1.70% to 2.40% group (P < 0.05), while TNF-α and IL-1β obtained minimum values in 1.70% group. In the kidney, the interleukin 10 (IL10) mRNA expression was significantly higher in 1.70% group than that in other groups, while the IL-1β expression in 1.70% group was on the contrary and significantly lower than that in 2.40% group (P < 0.05). Results of this study suggested that moderate dietary n-3 HUFA (1.47%-1.70% HUFA) could improve the growth performance, non-specific immunity and inhibit the inflammatory response of hybrid grouper.
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