文章摘要
饲料脂肪水平对大口黑鲈CPT1表达的影响
Effect of dietary lipid level on the expression of CPT1 in the Micropterus salmoides
投稿时间:2019-09-05  修订日期:2019-09-22
DOI:
中文关键词: 大口黑鲈  脂肪水平  CPT1  基因表达  qPCR
英文关键词: M.salmoides  lipid level  CPT1  gene expression  qPCR
基金项目:国家自然科学基金(31672659)
作者单位E-mail
宋铭琪 西南大学 1007691313@qq.com 
钟云飞 西南大学  
郭佳玲 西南大学  
陈拥军 西南大学  
罗莉 西南大学  
林仕梅 西南大学 linsm198@163.com 
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中文摘要:
      为研究脂肪水平对大口黑鲈鱼体组织中肉碱棕榈酰转移酶I (CPT1)基因表达的影响及不同生长阶段的表达规律。实验设计6%(低脂)、12%(中脂)和18%(高脂)3种脂肪水平的等氮不等脂饲料,饲养初始体质量为(23.60±1.26)g的大口黑鲈,分别在饲养第30、60和90 天取肝脏、肠道、肾脏和肌肉等组织样品,以18S为内参基因,用CPT1特异性引物进行实时荧光定量实验,使用2-△△ct法得到CPT1基因的相对表达数据。结果显示,CPT1基因在肝脏中表达量最高,在肠道和肾脏中次之,在鳃丝、心脏、肌肉、胃、脑以及脾脏中表达量较低;在不同脂肪水平处理下,CPT1表达量随脂肪水平的增加而增加;在不同生长阶段中,CPT1表达量随饲养时间的延长而增加。研究表明,饲料脂肪水平及饲养时间会诱导大口黑鲈组织CPT1基因的表达,表明CPT1可能参与机体脂肪的分解代谢。
英文摘要:
      To study the effect of lipid level on the expression of carnitine palmitoyl transferase I (CPT1) gene in the body tissues of largemouth bass and the expression patterns of different growth stages. Three different isonitrogenous diets with graded levels of lipid [6% (low lipid), 12% (medium lipid) and 18% (high lipid)] were feed to largemouth bass with the initial weight of 23.60 ± 1.26g. Tissue samples of liver, intestine, kidney and muscle were taken on the 30th, 60th and 90th day of feeding, and the 18S was used as the internal reference gene. The real-time PCR experiment was performed with CPT1 specific primers, and the relative expression data of CPT1 gene was obtained by 2-△△ct method. The results showed that the expression level of CPT1 gene was highest in the liver (P<0.05), followed by intestinal and kidney (P<0.05), and the expression level was lower in silk, heart, muscle, stomach, brain and spleen; Under different fat levels, the expression of CPT1 increased with the increase of lipid level; in different growth stages, the expression of CPT1 increased with the prolongation of feeding time. The results showed that the increase of lipid level and feeding time would induce the expression of CPT1 gene, and CPT1 gene may played a certain role in the decomposition of fat in the largemouth bass.
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