文章摘要
银离子长效杀菌效应的机制研究
The mechanism underlying prolonged antibacterial effect of silver ion
投稿时间:2019-09-07  修订日期:2019-12-13
DOI:
中文关键词: 银离子  长效杀菌机制  僵尸效应
英文关键词: silver ions  prolonged antibacterial mechanism  zombie effect
基金项目:
作者单位E-mail
资英娟 仲恺农业工程学院动物科技学院,广东省水环境与水产品安全工程技术研究中心 yingjuanzi@163.com 
李岩 仲恺农业工程学院动物科技学院,广东省水环境与水产品安全工程技术研究中心  
黎烽 仲恺农业工程学院动物科技学院,广东省水环境与水产品安全工程技术研究中心  
杨敏璇 仲恺农业工程学院动物科技学院,广东省水环境与水产品安全工程技术研究中心  
赵丽娟 仲恺农业工程学院动物科技学院,广东省水环境与水产品安全工程技术研究中心  
李凤麟 仲恺农业工程学院动物科技学院,广东省水环境与水产品安全工程技术研究中心  
秦真东 仲恺农业工程学院动物科技学院,广东省水环境与水产品安全工程技术研究中心  
林蠡 仲恺农业工程学院动物科技学院,广东省水环境与水产品安全工程技术研究中心 linli@zhku.edu.cn 
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中文摘要:
      由抗生素导致的耐药性问题日益严重,因此新型抗菌药物的研发迫在眉睫。银离子因其具有抗菌作用,而且还具有安全、无耐药性、稳定性高等优点而备受关注。为了探究银离子的抗菌机制,本试验选择几种常见水生细菌,研究其对银离子的耐受性和细菌种内和种间的“僵尸效应”(即被银离子杀死的细菌可以杀死其他新鲜的细菌),以进一步明确银离子长效杀菌的机理。细菌对银离子耐受实验发现,银离子对五种细菌都表现出了明显的生长抑制。同种细菌和异种细菌之间都表现出明显的“僵尸效应”,并且随着银离子浓度的提高,“僵尸效应”的效果越明显。为进一步研究银离子杀菌机制,通过透射电镜观察银离子处理后的嗜水气单胞菌和无乳链球菌,结果显示银离子处理后的细菌出现胞质皱缩,细胞膜呈弥散状态,甚至破裂,细胞内容物外流,最终致使细菌死亡。综上所述,本研究结果阐明了银离子长效杀菌的机制,并发现细菌之间存在的“僵尸效应”,为研发新型的抗菌药物提供参考。
英文摘要:
      Drug resistance mainly caused by the abuse of antibiotics is becoming more and more serious, therefore there is an urgent need to develop novel antibacterial agents. Due to its safety, non-resistance, and high stability, the antibacterial activity of silver ion(Ag+) has attracted the attention of more and more researchers. In order to explore the antibacterial mechanism of Ag+, several common aquatic bacteria, named (Escherichia coli, Aeromonas hydrophila, Streptococcus agalactiae, Bacillus subtilis, Edwardsiella ictaluri), were used to test the Ag+ tolerance or bactericidal activity and its "zombie effect" ( the bacteria killed by Ag+ can kill other fresh bacteria). The results of tolerance experiments of bacteria to Ag+ showed that Ag+ had significant growth inhibition on the all five bacteria. The higher concentration of Ag+, the more apparent "zombie effect" was observed between the same species of bacteria as well as different species of bacteria. To further investigate the mechanism underlying of the antibacterial activity of Ag+, the Ag+ treated A. hydrophila and S. agalactiae were observed by transmission electron microscopy (TEM). The TEM results demonstrated that Ag+ could cause the separation of the cytoplasmic membrane (CM) from the cell wall and discharge of cytoplasmic organelles, so as it could cause the lysis of the bacterial cell wall. In summary, this study reveals the prolonged antibacterial mechanism of Ag+ which will pave a new way for the development of novel antibacterial agents.
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