文章摘要
黑水虻油替代豆油对草鱼生长性能、抗氧化酶和肠道菌群的影响
Effect of replacing soybean oil with black soldier fly (Hermetia illucensS) larvae oil on the growth performance, anti-oxidant enzyme and intestinal microbiota of grass carp (Ctenopharyngodon idellus)
投稿时间:2019-09-09  修订日期:2019-09-26
DOI:
中文关键词: 草鱼  黑水虻  豆油  生长性能  抗氧化能力  肠道菌群组成
英文关键词: Grass carp  Hermetia illucens  soybean oil  growth performance  anti-oxidant activity  intestinal microbiota
基金项目:国家自然科学基金(31402311, 31673671和31872581);NSFC-河南联合基金(U1704109);河南省重点科技攻关项目(国家科技合作领域)(182102410031)
作者单位E-mail
陈延娜 河南师范大学 2934473423@qq.com 
卢荣华 河南师范大学  
杨国坤 河南师范大学  
张玉茹 河南师范大学  
秦超彬 河南师范大学  
吉红 西北农林科技大学动物科技学院  
聂国兴 河南师范大学 niegx@htu.cn 
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中文摘要:
      为研究黑水虻油替代豆油在草鱼生产实践中的效果,本实验以黑水虻油替代草鱼基础饲料中0(SO),25%(BSO25),50%(BSO50),75%(BSO75)和 100%(BSO100)的豆油并配制成5种等氮 (蛋白质水平为32%)等脂(脂肪水平为4.9%) 的饲料,饲喂草鱼[初始体质量(13.37±1.07g) ]56 d后检测生长性能、体成分、血清生化指标、抗氧化能力、肠道和肝脏组织结构及肠道菌群组成等变化。结果显示,各组之间草鱼增重率(WGR)、特定生长率(SGR)、饲料系数(FCR)、肥满度(CF)、脏体比(VSI)、肝体比(HSI)变化均不显著。与SO组相比,BSO25和BSO50组血清超氧化物歧化酶 (SOD) 活性显著增加,SM75和SM100组的丙二醛(MDA)含量均显著降低,BSO50、BSO75和BSO100组的过氧化氢酶 (CAT) 活性均显著增加。BSO100组肝细胞呈现为不规则形状,血清中AST、ALT及LDL-C 的水平均显著高于SO组。BSO100组群落多样性指数Sobs、Shannon和丰富度指数Chao、Ace显著高于SO组。研究表明,在本实验条件下,黑水虻油替代饲料中100%的豆油不会影响草鱼的生长,且可以提高草鱼抗氧化能力及肠道菌群的丰度和多样性,但在BSO100组中,草鱼肝脏受损,因此建议草鱼饲料中黑水虻油替代大豆油的量以不超过75%为宜。
英文摘要:
      In order to study the effects of replacement of soybean oil (SO) with black soldier fly larvae oil (BSO) in grass carp production practice, five isolipidic and isonitrogen experimental diets were formulated by replacing 0% (SO), 25% (BSO25), 50% (BSO50), 75% (BSO75) and 100% (BSO100) of SO with BSO, respectively. After 56 days of feeding, the changes of growth performance, body composition, serum biochemical index, antioxidant capacity, intestinal and liver tissue structure, and intestinal microbiota of grass carp were detected. The results showed that there were no significant differences in WGR, FCR, SGR, VSI, CF and HSI among the treatment groups. Compared with the SO group, the activity of serum superoxide dismutase (SOD) in BSO25 and BSO50 groups was significantly higher, malondialdehyde (MDA) activity in BSO75 and BSO100 groups was significantly decreased, and the catalase (CAT) activity was significantly increased in BSO50, BS075 and BSFO100 groups. The morphology of liver cells in BSO100 group was mostly of irregular shape, with unclear boundaries between cells. The enzyme activity of AST, ALT and LDL-C in serum was significantly higher than that of SO group. The community diversity index Sobs, Shannon and the richness index Chao, Ace in the BSO100 group was significantly higher than the SO group. The results showed that 100% soybean oil replaced by BSO did not affect the growth of grass carp, and it could enhance the antioxidant capacity and the abundance and diversity of intestinal microbiota., but damage occurred in the BSO100 group. Therefore, it is recommended that the amount of BSO instead of soybean oil should not exceed 75% in the grass carp diet.
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