文章摘要
凡纳滨对虾JAK和STAT基因在苏云金芽孢杆菌侵染过程中的表达变化分析
Relative expressive profile analysis of JAK and STAT genes in Litopenaeus vannamei after Bacillus thuringiensis challenge
投稿时间:2019-09-19  修订日期:2019-10-28
DOI:
中文关键词: 凡纳滨对虾  JAK/STAT  基因表达  细菌感染  天然免疫应答
英文关键词: Litopenaeus vannamei  JAK/STAT  gene expression  bacterial infection  innate immune response
基金项目:国家自然科学基金(31472299),天津市应用基础与前沿技术研究计划(15JCZDJC33800),天津市水产生态及养殖重点实验室开放基金(TJAE2015005),天津市高等学校创新团队建设规划(TD13-5076),天津市人才发展特殊支持计划高层次创新创业团队项目,天津市水产产业技术体系创新团队(ITTFRS2017007)
作者单位E-mail
李丹蕾 天津市动植物抗性重点实验室/天津师范大学生命科学学院 670294841@qq.com 
郝少彦 天津市动植物抗性重点实验室/天津师范大学生命科学学院  
张亦陈 天津市动植物抗性重点实验室/天津师范大学生命科学学院  
耿绪云 天津市水产研究所  
刘逸尘 天津市动植物抗性重点实验室/天津师范大学生命科学学院 liuychn@outlook.com;skylyc@tjnu.edu.cn 
孙金生 天津市动植物抗性重点实验室/天津师范大学生命科学学院  
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中文摘要:
      JAK/STAT信号通路作为天然免疫的重要组成部分,在免疫应答中发挥着至关重要的作用,近年来,甲壳动物JAK/STAT通路中的关键分子陆续已有初步报道,但其在病原感染过程中的精确功能尚不明确。苏云金芽孢杆菌是对虾肝胰腺坏死症的致病菌之一,本研究利用半定量PCR技术分析了凡纳滨对虾JAK (Lv-JAK)和STAT (Lv-STAT)基因的组织表达特征,并利用实时荧光定量PCR (qPCR)技术分析了其在苏云金芽孢杆菌侵染过程中的表达变化特征。结果显示:Lv-JAK和Lv-STAT基因在凡纳滨对虾的9种组织中有不同程度的表达,均在鳃、肠道和心脏中表达量较高。苏云金芽孢杆菌感染后,在鳃组织中,Lv-JAK和Lv-STAT基因的相对表达量在感染的中晚期(24hpi~72hpi)显著上调表达;在肠道组织中,Lv-JAK基因的相对表达量在感染后的6和24小时显著上调,Lv-STAT基因的相对表达量在感染后的24和72小时显著上调。上述研究表明JAK和STAT基因在一定程度上参与了凡纳滨对虾体内由苏云金芽孢杆菌引发的天然免疫应答过程,探讨对虾JAK和STAT基因应答苏云金芽孢杆菌感染的表达变化特征,将有助于研究对虾JAK/STAT通路在应答病原菌侵染过程中的功能和作用机制。
英文摘要:
      ABSTRACT: JAK/STAT signaling pathway, as an important part of innate immunity, plays an important role in immune response. In recent years, some key molecules in JAK/STAT pathway of crustaceans have been reported, but the precise function of JAK/STAT signaling pathway in pathogenic infection is still unclear. Bacillus thuringiensis is one of the main pathogens of shrimp hepatopancreatic necrosis. In this study, the tissue expression of JAK (Lv-JAK) and STAT (Lv-STAT) gene in Litopenaeus vannamei were analyzed by semi-quantitative PCR. The relative expression profile of Lv-JAK and Lv-STAT during the infection of Bacillus thuringiensis were analyzed by real-time fluorescence quantitative PCR (qPCR). The results showed that transcript of Lv-JAK and Lv-STAT was mainly detected in gill, intestine and heart. The relative expression of Lv-JAK and Lv-STAT were significantly up-regulated in gill tissues at the middle and late stages of B. thuringiensis infection (24hpi~72hpi). In intestine, the relative expression of Lv-JAK was significantly up-regulated at 6hpi and 24hpi, while that of Lv-STAT was significantly up-regulated at 24hpi and 72hpi. These studies indicated that JAK and STAT participated in the innate immune response induced by B. thuringiensis in L. vannamei. Understand the expression profile of JAK and STAT in response to B. thuringiensis infection will be helpful to study the function and regulatory mechanism of shrimp JAK/STAT signaling pathway in response to bacterial infection.
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