文章摘要
盐度胁迫对尼罗罗非鱼免疫相关指标的影响
Effects of salinity stress on immune-related parameters of the Nile tilapia (Oreochromis niloticus)
投稿时间:2019-09-22  修订日期:2019-11-17
DOI:
中文关键词: 盐度胁迫  罗非鱼  免疫相关指标
英文关键词: Oreochromis niloticus  salinity stress  immune-related parameters
基金项目:
作者单位E-mail
蔡然 有害生物控制与资源利用国家重点实验室 alan6382@163.com 
陈立敏 有害生物控制与资源利用国家重点实验室  
辛颖 有害生物控制与资源利用国家重点实验室  
赵早亚 有害生物控制与资源利用国家重点实验室  
俞筱筝 有害生物控制与资源利用国家重点实验室  
黄金凤 有害生物控制与资源利用国家重点实验室  
廖宗甄 有害生物控制与资源利用国家重点实验室  
李文笙 有害生物控制与资源利用国家重点实验室 lsslws@mail.sysu.edu.cn 
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中文摘要:
      为探究盐度胁迫对尼罗罗非鱼免疫的影响,作者对体质量(35±5)g的尼罗罗非鱼进行了急性和慢性的盐度胁迫实验,对免疫相关指标进行了检测和分析。在急性盐度胁迫中,设置0、5和15 三个盐度,分别在胁迫后6、12、24、48和96 h进行取样,检测血清SOD、CAT、GSH-Px和AKP的活性。在慢性实验中,设置0、10、20和30 四个盐度,胁迫8周后检测血清SOD、CAT、GSH-Px和AKP活性,并进行了无乳链球菌易感性实验。结果显示:①血清中SOD的活性在急性盐度胁迫6、12和24 h时都有随盐度上升而上升的趋势,但在96 h时盐度15组酶活性显著低于盐度5 组;在慢性盐度胁迫下各组呈现随着盐度升高酶活力显著性下降。②血清CAT在急性盐度胁迫下12和24 h时呈现随着盐度升高而显著性下降;在慢性胁迫下不存在显著性差异。③血清中GSH-Px活性在急性和慢性胁迫后,均呈现随着盐度升高而降低的趋势。④血清AKP活性在胁迫后6 h随盐度升高呈现显著性下降趋势;在慢性盐度胁迫下盐度20组显著低于其他实验组。⑤罗非鱼对无乳链球菌易感性实验中,盐度10组的易感性和0组之间无明显差异,盐度20和30组的易感性高于0组。本研究表明两种盐度胁迫均会引起免疫相关指标的变化:急性盐度胁迫实验表明5和15盐度的导致罗非鱼机体氧化损伤,但罗非鱼可以逐渐适应这一变化;慢性盐度胁迫实验表明高于20盐度会抑制罗非鱼多种免疫指标活性,造成对无乳链球菌易感性升高。
英文摘要:
      In order to explore the effects of salinity stress on the immunity of the Nile tilapia (Oreochromis niloticus), we conducted acute and chronic salinity stress experiments on tilapia with body weight of (35±5)g and some immune-related parameters of the serum were tested. In acute salinity stress, the salinities of 0, 5 and 15 were set, and the enzyme activities of SOD, CAT, GSH-Px and AKP in serum were detected by sampling at 6, 12, 24, 48 and 96 h after stress respectively. In the chronic experiment, four salinity levels of 0, 10, 20 and 30 were set, and the enzyme activity of SOD, CAT, GSH-Px and AKP in serum was detected after 8 weeks of stress. The results showed that: SOD activity in serum increased with salinity at 6, 12 and 24 h under acute salinity stress, but at 96h, enzyme activity in the salinities of 15 group was significantly lower than that in 5 group. Under chronic salinity stress, the enzyme activity of each group decreased significantly with the increase of salinity. CAT activity in serum decreased significantly with the increase of salinity at 12 h and 24h under acute salinity stress, and there was no significant difference after that. GSH-Px activity in serum decreased significantly with the increase of salinity at 6, 12 and 48 h after stress. AKP activity in serum significantly decreased with the increase of salinity at 6h after stress. In chronic the 20 group was significantly lower than the other group. In the experiment of susceptibility to Streptococcus agalatiae after chronic stress, the susceptibility of the salinities of 10 group to S. agalatiae had no obvious difference compared with 0, the susceptibility of 20 and 30 were higher than 0. This study showed that both kinds of stress could cause changes in immune-related indicators: acute salinity stress experiment showed that the salinities of 5 and 15 caused oxidative damage to the tilapia, but tilapia could gradually adapt to this change. Chronic salinity stress experiment showed that salinity above 20 could inhibit the activity of many immune indicators of tilapia, resulting in increased susceptibility to S. agalatiae.
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