文章摘要
青蛤(Cyclina sinensis)染色体制备及核型分析
Chromosome Preparation and Karyotype Analysis of Cyclaina sinensis
投稿时间:2019-10-16  修订日期:2019-12-24
DOI:
中文关键词: 青蛤  染色体  性染色体  单倍体  核型分析
英文关键词: Cyclaina sinensis  chromosome  heterosome  haploid  karyotype analysis
基金项目:国家贝类产业技术体系(CARS-49); 江苏省高等学校自然科学研究重大项目(18KJA240001); 中国水产科学研究院黄海水产研究所基本科研业务费(20603022018004) ; 江苏省渔业科技类项目(Y2018-27); 江苏省研究生科研与实践创新计划项目(No. SJCX190981, No.SJCX190979)
作者单位E-mail
段海宝 江苏省海洋生物资源与生态环境重点实验室 duanhaibao@126.com 
茆双 江苏省海洋生物资源与生态环境重点实验室  
陈义华 上海海洋大学水产种质资源发掘与利用教育部重点实验室  
葛红星 江苏省海洋生物资源与生态环境重点实验室  
魏敏 江苏省海洋生物资源与生态环境重点实验室  
周丽青 农业农村部海洋渔业可持续发展重点实验室 中国水产科学研究院黄海水产研究所 青岛
农业农村部海洋渔业可持续发展重点实验室 中国水产科学研究院黄海水产研究所 青岛 
 
符翔超 江苏省海洋生物资源与生态环境重点实验室  
孙泽鹏 江苏省海洋生物技术重点实验室  
董志国 江苏省海洋生物资源与生态环境重点实验室 dzg7712@163.com 
摘要点击次数: 38
全文下载次数: 0
中文摘要:
      以青蛤成体鳃组织和性成熟的雌、雄性腺为材料,采用常规制片方法,分别制备青蛤二倍体染色体和单倍体染色体,统计染色体数目,结果表明:青蛤二倍体与单倍体染色体数目分别为2n=38和n=19;核型分析发现,青蛤核型组成为2n=11m+6sm+2st,NF=76,未发现性染色体和随体染色体。本研究以青蛤成熟性腺为制备材料,成功制备单倍体染色体,与青蛤二倍体染色体互相印证,弥补了以前学者研究的不足,进一步丰富了对青蛤染色体数目及核型组成的认知。同时,也为青蛤染色体水平全基因组测序组装提供更加科学的基础资料。
英文摘要:
      The clam Cyclina sinensis is an economically important shellfish in China, it is urgent to explore the excellent germplasm resources of C. sinensis. This requires the support of accurate genetic and biological basic materials. In order to accurately understand the number of chromosome and its karyotype composition of C. sinensis. We prepared diploid chromosomes and haploid chromosomes from the gill tissue and mature gonad of C. sinensis, respectively. The results showed that the chromosome number of diploid was 2n = 38 and haploid was n = 19. On the basis of the above experiments, the well dispersed visual field of diploid chromosomes was measured by microphotography, and the karyotype was analyzed. The result of experiment indicates that the karyotype of C. sinensis was 2n = 11m + 6sm + 2st, NF = 76, and no heterosome and satellite chromosome were found. In this study, the haploid chromosome was successfully prepared by the mature gonads of the calm, which was to provide a new idea for molluscs chromosome studying. Moreover, The new discovery on chromosome number and karyotype of C. sinensis makes up for the results of previous studies, which was 2n =36. And it contribute to the more correct direction of phylogenetic research and genetic research of bivalve molluscs, also provides a more scientific basis for whole genome sequencing assembly of C. sinensis.
HTML     下载PDF阅读器
关闭

手机扫一扫看