文章摘要
王崇明.栉孔扇贝一种球形病毒的分离纯化及其超微结构观察[J].水产学报,2002,26(2):
栉孔扇贝一种球形病毒的分离纯化及其超微结构观察
Purification and ultrastructure of a spherical virus in cultured scallop Chlamys farreri
投稿时间:2014-04-10  修订日期:2014-04-10
DOI:
中文关键词: 栉孔扇贝 非包涵体 球形病毒 分离纯化 电镜观察 超微结构 支柱性产业 病毒粒子
英文关键词: Chlamys f arreri  non-occluded spherical viruses  isolat ion and purification  electronic observation
基金项目:国家重点基础研究发展计划(973计划)
作者单位地址
王崇明 中国水产科学研究院黄海水产研究所 中国水产科学研究院黄海水产研究所
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中文摘要:
      
英文摘要:
      Electron microscopic examinations of ultrathin sections reveal the presence of spherical virus-like particles in the digestive gland, kidney, and the intestine of moribund scallop Chlamys farreri collected during a severe mortality in Jiaozhou Bay ( Qingdao , China) in 2000. The virions are approximately 130 to 170 nm in diameter and have a bilaminal envelope, while the nucleocapsids are 90 - 140 nm. The virus occurs and distributes in the cytoplasmic vesicles of infected cells, and it is arranged in a scattered form without occluding protein. The viral part icles are consistent in size and morphology in preparations derived from the isolation and also from ultrathin sections. The spikes of envelope are clearly observed with negative staining. By the same methods, this virus was not found in the healthy scallop, indicating that this virus might be the causative aetio logy for the abnormal mortality.
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