文章摘要
刘小刚.微生态制剂对异育银鲫消化酶活性的影响[J].水产学报,2002,26(5):
微生态制剂对异育银鲫消化酶活性的影响
Effects of dietary Bacillus sp. and selenoyeast on activities of digestive enzymes of allogynogenetic crucian carp
投稿时间:2014-04-01  修订日期:2014-04-01
DOI:
中文关键词: 微生态制剂 酶活性 异育银鲫 芽孢杆菌 硒酵母 蛋白酶 淀粉酶 饲料添加剂
英文关键词: allogynogenetic crucian carp  Bacillus sp.  selenoyeast  protease  amylase
基金项目:上海自然科学基金资助项目(00ZC14054)
作者单位地址
刘小刚 上海水产大学渔业学院, 上海.. 200090 上海水产大学渔业学院, 上海.. 200090
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中文摘要:
      在基础饲料的基础上,分别添加0.1%、0.2%、0.4%芽孢杆菌,0.3%、0.6%、1.2%硒酵母,混合添加0.2%芽孢杆菌和0.3%、0.6%、1.2%硒酵母,制成9种微生态制剂饲料,以基础饲料为对照,在水泥地中饲养异育银鲫60d。然后,对异育银鲫肠道和肝胰脏的蛋白酶、淀粉酶进行了测定。结果表明,添加0.2%芽孢杆菌,异育银鲫肠道、肝胰脏蛋白酶活性分别比对照组提高466.2%和85.4%,添加0.1%芽孢杆菌,肠道肝胰脏淀粉酶活性分别比对照组提高83.7%和129.5%;添加硒酵母对肠道蛋白酶活性无显著影响,但添加0.6%硒酵母能使肝胰脏蛋白酶性比对照组提高43.7%,添加0.3%硒酵母能使肠道、肝胰脏淀粉酶活性分别比对照组提高84.2%和163.9%;同时添加0.2%芽孢杆菌和0.3%硒酵母能使肠道蛋白酶淀粉酶活性分别比对照组提高395.5%和95.4%,同时添加0.2%芽孢杆菌和0.3%、0.6%、1.2%硒酵母对肠、肝胰脏蛋白酶淀粉酶活性无显著影响。与单独添加0.2%芽孢杆菌相比,同时添加0.2%芽孢杆菌和硒酵母肠道、肝胰脏蛋白酶活性随硒酵母添加量的增加而显著降低,但淀粉酶活性两者之间差异不显著;与单独添加硒酵母相比,0.2%芽孢杆菌和硒酵母同时添加对肠道、肝胰脏蛋白酶、淀粉酶活性促进作用并不显著。
英文摘要:
      Allogynogemetic crucian carp ( average initial weight , 4. 2 .. 0. 3g ) were fed with basal diet supplemented 0. 1%, 0. 2%, 0. 4% Bacillus sp. , 0. 3%, 0. 6% , 1. 2% selenoyeast, complex of 0. 2% B . sp. and 0. 3%, 0. 6% , 1. 2% selenoyeast respectively for two months. Control group was fed with basal diet. Triplicate groups were made for each diet . The act ivit ies of protease and amylase in the intestine and hepatopancreas were determined. The activities of protease in the intestine and hepatopancreas fed with dietary 0. 2% B. sp. were 466. 2% and 85. 4% higher respectively than those of the control group. The activities of amylase in the intestine and hepatopancreas fed with dietary 0. 1% B . sp. were 83. 7% and 129. 5% higher respectively than those of the control group. The act ivit ies of protease in the intestine w ith three levels of selenoyeast were similar to those of the control group. The activity of protease in the hepatopancreas with dietary 0. 6% selenoyeast was 43. 7% higher than that of the control group, the act ivit ies of amylase in the intestine and hepatopancreas with dietary 0. 3% selenoyeast increased by 84. 2% and 163. 9% respectively. The activities of protease and amylase in the intestine fed with dietary 0. 2% B . sp. and 0. 3% selenoyeast complex were 395. 5% and 95. 4% higher respectively than those of the control group, while the activities of protease and amylase in the hepatopancreas fed with dietary 0. 2% B . sp. and three levels of selenoyeast were not remarkably higher than those of the control group. Compared to dietary 0. 2% B . sp. group, the activities of protease in the intestine and hepatopancreas fed w ith dietary 0. 2% B. sp. and three levels of selenoyeast complex decreased drastically w ith the increasement of the level of dietary selenoyeast, while there were no remarkable difference in the activities of amylase between two groups. Compared to dietary selenoyeast group, the activities of protease and amylase in the intestine and hepatopancreas fed with dietary selenoyeast and 0. 2% B . sp. complex were not changed remarkably.
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