文章摘要
朱树屏,刘恬敬,陈立人,周其芳,李复馨.温度及光照对浮游硅藻Nitzschia Closterium(Ehrenberg)W.Smith吸收P32的影响[J].水产学报,1965,2(1):53~58
温度及光照对浮游硅藻Nitzschia Closterium(Ehrenberg)W.Smith吸收P32的影响
NOTE ON THE INFLUENCES OF TEMPERATURE AND LIGHT ON THE ABSORPTION OF P32 BY A PLANKTONIC DIATOM NITZSCHIA CLOSTEBIUM(EHRENBERG)W,SMITH
  
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中文关键词: 群落  相似性指数  多样性指数  聚类分析  树状图  底层鱼类  深海  东海
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作者单位
朱树屏 水产部海洋水产研究所 
刘恬敬 水产部海洋水产研究所 
陈立人 水产部海洋水产研究所 
周其芳 水产部海洋水产研究所 
李复馨 水产部海洋水产研究所 
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中文摘要:
      据本所黄河口海区浮游植物的调查,海水中营养元素含量的变化,对控制浮游植物的数量有肯定作用,水温及光照对这种控制作用,又有重要的影响;并且这种影响因浮游植物的种类而有显著的差异(朱树屏、康元德,1962)。气候因子对各种浮游植物吸收营养元素的影响,尚缺确切资料,本文只简报温度及光照对P32被吸收的影响。
英文摘要:
      Subculture from unialgal culture of Nitaschia closterium,kept in our laboratory for manyyears, was made first in enriched sea water under suitable conditions for a week to gethealthy diatoms for inoculum. These diatoms were then carefully washed by centrifugingand subcultured in sterilized natural sea water,not enriched and very deficient in phosphate,with8 ×106 to 10×106 cells per ml., for ten hours before inoculating the cultures for experi-ments. Dead cells occurred if this starving culture lasted too long or contained too many cells.Experiments were all carried out in 500 ml. conical flasks each containing 200 ml. sterilizednatural sea water enriched with NO_3-N, 4 p.p.m. and P32 20μc. Cultures of cach serieswere illuminated by a 1,000 W.bulb.Diatoms were washed 5 times by centrifuging to get ridof the P32 adsorbed on the diatom frustules, before P32 absorbed being measured by a Geigercounter. 1. Influences of different temperatures on the absorption of P32. Cultures kept at 0, 5, 10, 15, 20, 25 and 30℃, were arranged obliquely under a 1,000W.bulb at a distance of 53 cm.measured from the surface of the bulb to the center of the bot-tom of the culture flask situated just inside a wall of white glazed paper which was usedto reflect the light. All cultures lasted 12 hours. Diatom cells in cultures at 15℃ absorb largest amount of P33 (Fig. 1,A), the increase ofdiatom numbers here is also the largest (Fig. 1, B), being up to 44.1%, and the amountof P32 absorbed by a single diatom cell is the largest as well. The next come in order arecultures at 20℃ and 10℃, the smallest absorption occurring in cultures at 5℃ and 0℃. 2. Influence of different illuminating periods on the absorption of P32. Cultures were kept at 15℃ and arranged straight beneath the 1,000 W.bulb with a dis-tance of 33. 5 cm. with light reflecting arrangement; all lasted 26 hours. Three series ofcultures, inoculated respectively with 6×106, 8×106 and 9×106 cells per ml., were made,each consisting of 4 groups, A, B, C and D: A. 26 hours' illumination. The absorption of P32 in 5 minutes gives a count of 100 per minute, increased to morethan 2,000 per minute in 8-9 hours, and then the absorption rate increases rapidly up to 12,800 counts per millute in 17 hours for cultures inoculated with 6×106 cells per ml. (Fig.2a,A), and up to 12,000 and 8,600 counts per minute in 20 and 21.5 hours for culturesinoculated with 8×106 and 9×106 cells per ml.respectively (Figs.2b, A 2c, A).The summitof the absorption curve is higher and reached earlier in cultures with a smaller inoculum. B. 14 hours' illumination followed by 12 hours' darkness. During the period of illumination the absorption curve (Fig. 2, B) follows more or lessthe course of the curve for 26 hours' illumination (Fig. 2,A). When the black-out starts,the absorption curve of cultures inoculated with 6×106 cells per ml. begins to drop im-mediately (Fig. 2a, B), while the curves of cultures inoculated with 8×106 and 9×106cells per ml.still going upward for another three and six hours respectively before dropping(Fig. 2 b, B 2 c, B). C. 12 hours' illumination after 14 hours' darkness. The absorption is very weak during the 14 hours' black-out period, giving only 100-200counts per minute and gradually increases after illumination being started, reaching gteatspeed after 6 hours' illumination (Fig. 2, C). D. 26 hours' black-out. Absorption is very weak throughout the 26 hours, being only 100-200 counts perminute. The following phenomena are worth noticing in the above illumination experiments: 1. Greatest absorption speed of P32 generally occure 8-12 (14) hours after illuminationbeing started. 2. The summit of the absorption curve is reached earlier in the culture with smaller ino-culum, possibly as a result of less sheltering effect and hence more light being received byeach cell. 3. The absorption curve drops after the summit is resched, as a result of P32 being se-creted out into the culture medium.
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