文章摘要
梁前进.中国几个金鱼品系血清抗原性的探讨[J].水产学报,1998,22(1):
中国几个金鱼品系血清抗原性的探讨
AN EXPLORATION ON THE ANTIGENICITY OF THE SERUM OF SEVERAL STRAINSOF GOLDFISHES FROM CHINA
投稿时间:2014-05-07  修订日期:2014-05-07
DOI:
中文关键词: 金鱼 血清 抗原性 电泳
英文关键词: Carassius auratus Var ., Serum,Ant igenicity ,Electrophorysis
基金项目:
作者单位地址
梁前进 北京师范大学生物系 北京师范大学生物系
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中文摘要:
      利用醋酸纤维薄膜电泳、SDS-聚丙烯酰胺凝胶电泳比较了鲫和几个金鱼品系(金鲫、草金鱼、红文鱼、红龙睛和红头蛋鱼)的血清蛋白成分,并用兔抗鲫血清对这几个品系进行了双向免疫扩散和对流免疫电泳分析。结果表明:⑴鲫和几个金鱼品系的血清蛋白成分及抗原性在很大程度上相似;⑵对电泳图谱的详细分析以及用高效价抗血清测定可以看出它们之间还存在不同程度的差别,并与演化地位相当一致。
英文摘要:
      Cellulose acetate membr ane and SDS -polyacr ylamide gel elect rophoresis wer e used to compare the serum components among crucian car p and several stra ins of goldfishes (gold cr ucian , gr ass goldfish , r ed wen goldfish , red dr agon-eye goldfish and red oval goldf ish )from China .Furthermore , these str ains wer e analysed by dual immune dif fusion and convect ive immune elect rophoresis in which ant i-crucian car p serum f rom rabbi t was used .The r esults are as follows :⑴ The serum components and an tigenicity of cr ucian car p and these st rains of goldf ishes are similar in a high degr ee .⑵ It is demonst rated that there ar e some differences in these fishes by the analysis of electrophore tic gr ams and the determination with highly eff icient ant i-serum.These dif ferences conside rably are consistent with the evolut ionary posit ions among these fishes .
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