文章摘要
姚纪花.我国六个地区银鲫种群线粒体DNA多态性的研究[J].水产学报,1998,22(4):
我国六个地区银鲫种群线粒体DNA多态性的研究
A STUDY ON MITOCHONDRIAL DNA POLYMORPHIS AMONG SIX GEOGRAPHICAL POPULATIONSOF CARASSIUS AURATUS GIBELIO IN CHINA
投稿时间:2014-05-06  修订日期:2014-05-06
DOI:
中文关键词: 银鲫 种群 线粒体DNA 限制性片段长度 多态性
英文关键词: Carassius auratus gibelio , Populat ion , mtDNA , RFLP
基金项目:
作者单位地址
姚纪花 上海水产大学渔业学院 上海水产大学渔业学院
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中文摘要:
      本文用一对引物对我国6个地区的银鲫进行mtDNA-ND5/6片段的PCR扩增,扩增产物(约2.5Kb)用6种限制性内切酶进行限制性片段长度多态性(RFLP)分析。共获得11种图谱和7种限制性类型(基因型)。根据各群体间的遗传距离构建了UPG聚类关系图。结果表明,6个银鲫种群中,云南群体与贵州群体的关系以及黑龙江与安徽群体之间的关系最近,它们分别归为一个群体;河南群体相对独立,它与黑龙江和安徽群体的遗传关系较近;江西群体与各群体间的亲缘关系最远。 更多还原
英文摘要:
      In six geogr aphical populat ions of Carassius auratus gibelio , the ND 5/6 r egion (approximately 2 .5Kb)of mtDNA genome have been amplif ied by the polymerase ch ain reaction (PCR), and the pr oducts were examined using rest rict ion fr agment length polymor phism (RFLP).11 elect rophoresis patterns and 7 haplotypes (genotypes)have been observed .Based on the genet ic distances , the UPG molecular phylogenet ic tr ee of the populat ions was constructed .The results indicate th at the six popula tions of the C .auratus gibelio can be imcorporated into four populat ions , namely , the Heilongjiang and Anhui , the Henan , the Yunnan and Guizhou , and the Jiangxi .The first is the closest to the third . The He nan is much closer to the Heilongjiang and Anhui .The far thest is between the Jiangxi and the other populat ions .
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